Modulation of intestinal and liver fatty acid-binding proteins in Caco-2 cells by lipids, hormones and cytokines

被引:38
作者
Dubé, N
Delvin, E
Yotov, W
Garofalo, C
Bendayan, M
Veerkamp, JH
Levy, E
机构
[1] Univ Montreal, Hop St Justine, Ctr Rech, Montreal, PQ H3T 1C5, Canada
[2] Univ Montreal, Dept Nutr, Montreal, PQ H3T 1C5, Canada
[3] Univ Montreal, Dept Biochem, Montreal, PQ H3T 1C5, Canada
[4] Univ Montreal, Dept Pathol & Cell Biol, Montreal, PQ H3T 1C5, Canada
[5] Univ Montreal, Dept Pediat, Montreal, PQ H3T 1C5, Canada
[6] Univ Nijmegen, Dept Biochem, Nijmegen, Netherlands
关键词
fatty acids; phosphatidylcholine; hydrocortisone; insulin; cytokines; fat absorption;
D O I
10.1002/jcb.1090
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Intestinal and liver fatty acid binding proteins (I- and L-FABP) are thought to play a role in enterocyte fatty acid (FA) trafficking. Their modulation by cell differentiation and various potential effecters was investigated in the human Caco-2 cell line. With the acquisition of enterocytic features, Caco-2 cells seeded on plastic progressively increased L-FABP quantities, whereas I-FABP was not detectable even very late in the maturation process. On permeable filters that improved differentiation markers (sucrase, alkaline phosphatase, transepithelial resistance), Caco-2 cells furthered their L-FABP content and expressed I-FABP. Western blot analysis showed a significant increase in I- and L-FABP expression following an 8-hour incubation period with butyric acid, oleic acid, and phosphatidylcholine. However, in all cases, I-FABP levels were higher than L-FABP concentrations regardless of the lipid substrates added. Similarly, hydrocortisone and insulin enhanced the cellular content of I- and L-FABP whereas leptin triggered I-FABP expression only after an 8-hour incubation. Finally, tumor necrosis factor-alpha was more effective in increasing the cytosolic amount of I-FABP levels. In conclusion, our data demonstrate that I-FABP expression is limited to fully differentiated Caco-2 cells and can be more easily regulated than L-FABP by lipids, hormones, and cytokines. J. Cell. Biochem. 81:613-620, 2001. (C) 2001 Wiley-Liss, Inc.
引用
收藏
页码:613 / 620
页数:8
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