Overlap and Effective Size of the Human CD8+ T Cell Receptor Repertoire

被引：306

作者：

Robins, Harlan S. ^{[1
]}

Srivastava, Santosh K. ^{[1
]}

Campregher, Paulo V. ^{[2
]}

Turtle, Cameron J. ^{[2
]}

Andriesen, Jessica ^{[1
]}

Riddell, Stanley R. ^{[2
]}

Carlson, Christopher S. ^{[3
]}

Warren, Edus H. ^{[1
]}

机构：

[1] Fred Hutchinson Canc Res Ctr, Program Computat Biol, Seattle, WA 98109 USA

[2] Fred Hutchinson Canc Res Ctr, Program Immunol, Seattle, WA 98109 USA

[3] Fred Hutchinson Canc Res Ctr, Program Canc Prevent, Seattle, WA 98109 USA

来源：

SCIENCE TRANSLATIONAL MEDICINE | 2010年 / 2卷 / 47期

关键词：

ANTIGEN RECEPTOR; RESPONSES;

D O I：

10.1126/scitranslmed.3001442

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Diversity in T lymphocyte antigen receptors is generated by somatic rearrangement of T cell receptor (TCR) genes and is concentrated within the third complementarity-determining region 3 (CDR3) of each chain of the TCR heterodimer. We sequenced the CDR3 regions from millions of rearranged TCR beta chain genes in nave and memory CD8(+) T cells of seven adults. The CDR3 sequence repertoire realized in each individual is strongly biased toward specific V-beta-J(beta) pair utilization, dominated by sequences containing few inserted nucleotides, and drawn from a defined subset comprising less than 0.1% of the estimated 5 x 10(11) possible sequences. Surprisingly, the overlap in the naive CD8(+) CDR3 sequence repertoires of any two of the individuals is similar to 7000-fold larger than predicted and appears to be independent of the degree of human leukocyte antigen matching.

引用

页数：9

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