Analysis of intact bacteriohopanepolyols from methanotrophic bacteria by reversed-phase high-performance liquid chromatography-atmospheric pressure chemical ionisation mass spectrometry

被引：111

作者：

Talbot, HM

Watson, DF

Murrell, JC

Carter, JF

Farrimond, P

机构：

[1] Univ Newcastle Upon Tyne, NRG, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England

[2] Univ Warwick, Dept Biol Sci, Coventry CV4 7AL, W Midlands, England

[3] Univ Bristol, Sch Chem, Organ & Biol Chem Sect, Bristol BS8 1TS, Avon, England

来源：

JOURNAL OF CHROMATOGRAPHY A | 2001年 / 921卷 / 02期

关键词：

bacteria; bacteriohopanepolyols; hopanoids; triterpenoids; terpenoids;

D O I：

10.1016/S0021-9673(01)00871-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Direct detection of most intact biohopanoids is not possible using conventional GC-MS techniques due to their highly functionalised and amphiphilic nature. Here we report the application of a new reversed-phase high-performance liquid chromatography method for the direct analysis of acetylated, intact bacteriohopanepolyols in solvent extracts of methanotrophic bacteria. Atmospheric pressure chemical ionisation mass spectrometric detection provides structural information relating to the number and types of functional groups present in the four biohopanoids detected: bacteriohopanetetrol, aminobacteriohopanetriol, -tetrol and -pentol. The method should facilitate the assessment of hopanoid composition of both bacteria and environmental samples. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：175 / 185

页数：11

共 19 条

[1] BACTERIOHOPANETETROL - ABUNDANT LIPID IN FRANKIA CELLS AND IN NITROGEN-FIXING NODULE TISSUE [J].