Phenolic composition in grape(Vitis vinifera L. cv. Malbec) ripened with different solar UV-B radiation levles by capillary zone electrophoresis

被引：91

作者：

Berli, Federico ^{[1
,2
]}

D'Angelo, Jose ^{[3
,4
]}

Cavagnaro, Bruno ^{[1
,2
]}

Bottini, Ruben ^{[1
,2
]}

Wuilloud, Rodolfo ^{[2
,5
]}

Silva, M. Fernanda ^{[1
,2
]}

机构：

[1] Natl Univ Cuyo, Fac Agron Sci, RA-5507 Mendoza, Argentina

[2] Consejo Nacl Invest Cient & Tecn, RA-1033 Buenos Aires, DF, Argentina

[3] Univ Nacl Cuyo, Inst Ciencia Basicas, Area Quim, RA-5500 Mendoza, Argentina

[4] Consejo Nacl Invest Cient & Tecn, CRICYT, IANIGLA, RA-5500 Mendoza, Argentina

[5] Consejo Nacl Invest Cient & Tecn, CRICYT, LISAMEN, RA-5500 Mendoza, Argentina

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2008年 / 56卷 / 09期

关键词：

phenolic composition; UV-B radiation; grapevines; Vitis vinifera L. cv. Malbec; capillary zone electrophoresis;

D O I：

10.1021/jf073421+

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

The responses of Vitis vinifera L. cv. Malbec to different solar ultraviolet-B radiation (UV-B) levels were assessed in two contrasting situations, under sunlight with full UV-B (+UV-B) and filtered UV-B (-UV-B), in three different locations at 500, 1000, and 1500 m above sea level (asl). To evaluate the effects of radiation, a simple, accurate, and rapid method for the separation and simultaneous determination of representative phenolic compounds in grape berry skins by capillary zone electrophoresis was developed. Separation was carried out in less than 20 min with 20 mM sodium tetraborate buffer containing 30% methanol, pH 9.00. The procedure is fast and reliable, and extracted grape berry skins can be directly analyzed without prior sample cleanup procedure. Berry skins from the +UV-B treatment at 1500 m asl showed the highest levels of total polyphenols anthocyanins, and resveratrol, compared with the -UV-B treatment at this altitude.

引用

页码：2892 / 2898

页数：7

共 38 条

[1] Stilbene content of mature Vitis vinifera berries in response to UV-C elicitation [J].

Adrian, M ;

Jeandet, P ;

Douillet-Breuil, AC ;

Tesson, L ;

Bessis, R .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2000, 48 (12) :6103-6105

[2]

[Anonymous], 2006, HDB ENOLOGY

[3] Separation of nonylphenol ethoxylates and nonylphenol by non-aqueous capillary electrophoresis [J].

Babay, PA ;

Gettar, RT ;

Silva, MF ;

Thiele, B ;

Batistoni, DA .

JOURNAL OF CHROMATOGRAPHY A, 2006, 1116 (1-2) :277-285

[4]

Beggs Christopher J., 1994, P733

[5] Molecular events following perception of ultraviolet-B radiation by plants [J].

Brosché, M ;

Strid, Å .

PHYSIOLOGIA PLANTARUM, 2003, 117 (01) :1-10

[6] Variations in the profile and content of anthocyanins in wines made from Cabernet Sauvignon and hybrid grapes [J].

Burns, J ;

Mullen, W ;

Landrault, N ;

Teissedre, PL ;

Lean, MEJ ;

Crozier, A .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2002, 50 (14) :4096-4102

[7] Effect of postharvest ultraviolet irradiation on resveratrol and other phenolics of cv. Napoleon table grapes [J].

Cantos, E ;

García-Viguera, C ;

de Pascual-Teresa, S ;

Tomás-Berberán, FA .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2000, 48 (10) :4606-4612

[8] Determination of phenolic compounds in grape skin by capillary electrophoresis with simultaneous dual fluorescence and diode array absorption detection after dynamic superheated liquid leaching [J].

Capote, Feliciano Priego ;

Luque Rodriguez, Jose Manuel ;

Luque de Castro, Maria Dolores .

JOURNAL OF CHROMATOGRAPHY A, 2007, 1139 (02) :301-307

[9] Gene expression profiling in response to ultraviolet radiation in maize genotypes with varying flavonoid content [J].

Casati, P ;

Walbot, V .

PLANT PHYSIOLOGY, 2003, 132 (04) :1739-1754

[10] Cloud point preconcentration prior to capillary zone electrophoresis:: Simultaneous determination of platinum and palladium at trace levels [J].

Cerutti, S ;

Silva, MF ;

Gásquez, JA ;

Olsina, RA ;

Martínez, LD .

ELECTROPHORESIS, 2005, 26 (18) :3500-3506

← 1 2 3 4 →