Basal and metal-induced expression of metallothionein isoform 1 and 2 genes in the RWPE-1 human prostate epithelial cell line

The human prostate gland has low basal expression of the metallothionein-1 and -2 proteins. In prostate cancer, MT-1/2 protein expression is variable and correlates directly with the increasing Gleason score of the tumor. The goal of the present study was to determine if the RWPE-1 cell line is a good model to elucidate the mechanisms underlying the alterations in MT-1/2 expression that occur during the development of prostate cancer. It was shown that the RWPE-1 cell line and in situ prostate tissue have identical expression profiles of MT-1 and MT-2 isoform-specific mRNAs (MT-1E, MT-1X and MT-2A) and similar levels of MT-1/2 protein. It was also shown that the RWPE-1 cells respond to Zn+2 and Cd+2 exposure by induction of the basally expressed NIT mRNAs and the accumulation of high levels MT-1/2 protein (in excess of 10% of total protein). It was also shown that additional MT-1 mRNAs were expressed when the cells were exposed to either metal; MT-1A, MT-1F, MT-G and MT-1H for Cd+2-exposed cells; and, NIT-IF, NIT-G and MT-1H for Zn+2-exposed cells. The results suggest that RWPE-1 cells may be a valuable system to define the interplay between Zn+2 concentration, Cd+2 exposure and NIT in normal and malignant prostate epithelial cells. Copyright (C) 2007 John Wiley & Sons, Ltd.