Potential live vaccines for HIV

被引:7
作者
Burnett, MS [1 ]
Wang, N [1 ]
Hofmann, M [1 ]
Kitto, GB [1 ]
机构
[1] Univ Texas, Inst Mol & Cellular Biol, Dept Chem & Biochem, Austin, TX 78712 USA
基金
美国国家卫生研究院;
关键词
salmonella; reverse transcriptase; mucosal IgA;
D O I
10.1016/S0264-410X(00)00303-0
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Potential live vaccines for HIV were developed using an Lpp-OmpA system to target an HIV antigen, reverse transcriptase, or an immunodominant epitope of this enzyme, to the outer membrane of an attenuated strain of Salmonella SL3261. These live vaccines were administered orally to mice, and fecal IgA and helper T cell responses were measured. Results indicated a fecal IgA response specific to HIV reverse transcriptase, as well as a reverse transcriptase-specific helper T cell response, as measured by proliferation assays. Additionally, tests with the epitope vaccines showed a selective cytotoxic CD8 T cell response. These results suggest that this method of antigen targeting to the outer membrane of attenuated bacterial vectors is very promising not only for HIV vaccine development, but also for antigens from other viral or bacterial pathogens, which could be inserted into the Lpp-OmpA protein construct, to elicit mucosal IgA and T cell responses. (C) 2000 Published by Elsevier Science Ltd.
引用
收藏
页码:735 / 742
页数:8
相关论文
共 24 条
  • [1] Bergmann CC, 1996, J IMMUNOL, V157, P3242
  • [2] GENE-EXPRESSION AND THE DEVELOPMENT OF LIVE ENTERIC VACCINES
    CHARLES, I
    DOUGAN, G
    [J]. TRENDS IN BIOTECHNOLOGY, 1990, 8 (05) : 117 - 121
  • [3] ISOLATION OF A NEW HUMAN RETROVIRUS FROM WEST-AFRICAN PATIENTS WITH AIDS
    CLAVEL, F
    GUETARD, D
    BRUNVEZINET, F
    CHAMARET, S
    REY, MA
    SANTOSFERREIRA, MO
    LAURENT, AG
    DAUGUET, C
    KATLAMA, C
    ROUZIOUX, C
    KLATZMANN, D
    CHAMPALIMAUD, JL
    MONTAGNIER, L
    [J]. SCIENCE, 1986, 233 (4761) : 343 - 346
  • [4] CLERICI M, 1997, VACCINE WEEKLY 0602, P7
  • [5] CURTISS R, 1989, CURR TOP MICROBIOL, V146, P35
  • [6] Oral somatic transgene vaccination using attenuated S-typhimurium
    Darji, A
    Guzman, CA
    Gerstel, B
    Wachholz, P
    Timmis, KN
    Wehland, J
    Chakraborty, T
    Weiss, S
    [J]. CELL, 1997, 91 (06) : 765 - 775
  • [7] DOUGAN G, 1989, ADV VET SCI COMP MED, V33, P271
  • [8] TRANSPORT AND ANCHORING OF BETA-LACTAMASE TO THE EXTERNAL SURFACE OF ESCHERICHIA-COLI
    FRANCISCO, JA
    EARHART, CF
    GEORGIOU, G
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (07) : 2713 - 2717
  • [9] PRACTICAL APPLICATIONS OF ENGINEERING GRAM-NEGATIVE BACTERIAL-CELL SURFACES
    GEORGIOU, G
    POETSCHKE, HL
    STATHOPOULOS, C
    FRANCISCO, JA
    [J]. TRENDS IN BIOTECHNOLOGY, 1993, 11 (01) : 6 - 10
  • [10] GENOME ORGANIZATION AND TRANSACTIVATION OF THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-2
    GUYADER, M
    EMERMAN, M
    SONIGO, P
    CLAVEL, F
    MONTAGNIER, L
    ALIZON, M
    [J]. NATURE, 1987, 326 (6114) : 662 - 669