Nuclear DNA contents of Phalaenopsis sp and Doritis pulcherrima

被引：47

作者：

Lin, S

Lee, HC

Chen, WH

Chen, CC

Kao, YY

Fu, YM

Chen, YH

Lin, TY ^{[1
]}

机构：

[1] Natl Tsing Hua Univ, Dept Life Sci, Hsinchu 30043, Taiwan

[2] Taiwan Sugar Res Inst, Dept Hort, Tainan 701, Taiwan

[3] Natl Taiwan Univ, Dept Bot, Taipei 10764, Taiwan

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE | 2001年 / 126卷 / 02期

关键词：

Orchidaceae; endoreduplication; flow cytometry; genome size;

D O I：

10.21273/JASHS.126.2.195

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

Nuclear DNA contents were estimated by flow cytometry in 18 Phalaenopsis Blume species and Doritis pulcherrima Lindl. DNA amounts differed 6.07-fold, from 2.74 pg/diploid nuclear DNA content (2C) in P. sanderiana Rchb.f. to 16.61 pg/2C in P. parishii Rchb.f. Nuclear DNA contents of P. aphrodite Rchb.f. clones, W01-38 (2n = 2x = 38), W01-41 (2n = 3x = 57), and W01-22 (2n = 4x = 76), displayed a linear relationship with their chromosome numbers, indicating the accuracy of flow cytometry. Our results also suggest that the 2C-values of the Phalaenopsis sp. correlate with their chromosome sizes. The comparative analyses of DNA contents may provide information to molecular geneticists and systematists for genome analysis in Phalaenopsis. Endoreduplication was found in various tissues of P. equestris at different levels. The highest degree of endoreduplication in P. equestris was detected in leaves.

引用

页码：195 / 199

页数：5

共 24 条

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