Osthole Regulates Inflammatory Mediator Expression through Modulating NF-κB, Mitogen-Activated Protein Kinases, Protein Kinase C, and Reactive Oxygen Species

被引：108

作者：

Liao, Pei-Chun ^{[2
]}

Chien, Shih-Chang ^{[3
]}

Ho, Chen-Lung ^{[4
]}

Wang, Eugene L-Chen ^{[4
]}

Lee, Shu-Ching ^{[4
]}

Kuo, Yueh-Hsiung ^{[5
]}

Jeyashoke, Narumon ^{[6
]}

Chen, Jie

Dong, Wei-Chih ^{[2
]}

Chao, Louis Kuoping ^{[1
]}

Hua, Kuo-Feng ^{[2
,7
]}

机构：

[1] China Med Univ, Dept Cosmecem, Taichung, Taiwan

[2] Natl Ilan Univ, Inst Biotechnol, Ilan, Taiwan

[3] China Med Univ, Sch Chinese Med Resources, Taichung, Taiwan

[4] Taiwan Forestry Res Inst, Div Wood Cellulose, Taipei, Taiwan

[5] China Med Univ, Tsuzuki Inst Tradit Med, Coll Pharm, Taichung, Taiwan

[6] King Mongkuts Univ Technol Thonburi, Sch Bioresources & Technol, Bangkok, Thailand

[7] China Med Univ, Grad Inst Drug Safety, Taichung, Taiwan

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2010年 / 58卷 / 19期

关键词：

Osthole; LPS; inflammation; signaling; CNIDIUM-MONNIERI; IN-VITRO; CELLS; PATHWAY; INHIBITION; MACROPHAGES; SUPPRESSES; SECRETION; HEPATITIS; FRUITS;

D O I：

10.1021/jf102812t

中图分类号：

S [农业科学];

学科分类号：

082806 [农业信息与电气工程];

摘要：

Osthole, a coumarin compound, has been reported to exhibit various biological activities; however the cellular mechanism of its immune modulating activity has not yet been fully addressed. In this study we isolated osthole from the seeds of Cnidium monnieri and demonstrated that osthole inhibited TNF-alpha, NO and COX-2 expression in LOS-stimulated macrophages, without reducing the expression of IL-6. Furthermore, the phosphorylation of p38, JNK1/2, PKC-alpha and PKC-epsilon induced by LIDS was inhibited by osthole; however, the phosphorylation of ERK1/2 and PKC-delta was not reduced by osthole. Osthole also inhibited NF-kappa B activation and ROS release in LOS-stimulated macrophages. Our current results indicated that osthole is the major anti-inflammatory ingredient of Cnidium monnieri seed ethanol extract.

引用

页码：10445 / 10451

页数：7

共 29 条

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IκB-NF-κB structures:: At the interface of inflammation control [J].