Molecular dissection of a Bifidobacterial replicon

被引：25

作者：

Cronin, Michelle ^{[1
]}

Knobel, Moritz ^{[1
]}

O'Connell-Motherway, Mary ^{[1
]}

Fitzgerald, Gerald F. ^{[1
]}

van Sinderen, Douwe ^{[1
]}

机构：

[1] Natl Univ Ireland Univ Coll Cork, Alimentary Pharmabiot Ctr, Dept Microbiol, Cork, Ireland

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2007年 / 73卷 / 24期

关键词：

D O I：

10.1128/AEM.01630-07

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The 2.1-kb cryptic plasmid pCIBAO89 from Bifidobacterium asteroides harbors a 1.4-kb segment which is sufficient for its autonomous replication. The segment is divided into two parts, the presumed replication origin, ori89, and the rep gene encoding the putative 41-kDa Rep89 replication initiation protein. This minimal replication region of pCIBAO89 was functionally dissected by transcriptional analyses as well as by DNA-binding studies, and the information obtained was exploited to create a number of Escherichia coli-Bifidobacterium shuttle vectors capable of transforming various bifidobacteria with an efficiency of up to 106 transformants/mu g DNA.

引用

页码：7858 / 7866

页数：9

共 63 条

[1] BASIC LOCAL ALIGNMENT SEARCH TOOL [J].

ALTSCHUL, SF ;

GISH, W ;

MILLER, W ;

MYERS, EW ;

LIPMAN, DJ .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410

[2] Screening for plasmids among human bifidobacteria species:: Sequencing and analysis of pBC1 from Bifidobacterium catenulatum L48 [J].

Alvarez-Martin, Pablo ;

Belen Florez, Ana ;

Mayo, Baltasar .

PLASMID, 2007, 57 (02) :165-174

[3]

[Anonymous], 1989, Molecular Cloning

[4] A convenient and reproducible method to genetically transform bacteria of the genus Bifidobacterium [J].

Argnani, A ;

Leer, RJ ;

vanLuijk, N ;

Pouwels, PH .

MICROBIOLOGY-UK, 1996, 142 :109-114

[5] Isolation and sequencing of the replication region of plasmid pBFp1 isolated from a marine biofilm [J].

Bergström, M ;

Hermansson, M ;

Dahlberg, C .

PLASMID, 2004, 51 (03) :179-184

[6] Molecular characterization of three plasmids from Bifidobacterium longum [J].

Corneau, N ;

Émond, É ;

LaPointe, G .

PLASMID, 2004, 51 (02) :87-100

[7]

DE MAN J. C., 1960, JOUR APPL BACT, V23, P130, DOI 10.1111/j.1365-2672.1960.tb00188.x

[8] Identification and nucleotide sequence analysis of a cryptic plasmid, pRM21, from Rhodothermus marinus [J].

Ernstsson, S ;

Bjornsdottir, SH ;

Jónsson, ZO ;

Thorbjarnardottir, SH ;

Eggertsson, G ;

Palsdottir, A .

PLASMID, 2003, 49 (02) :188-191

[9] Bifidobacterium longum as an oral delivery system of endostatin for gene therapy on solid liver cancer [J].

Fu, GF ;

Li, X ;

Hou, YY ;

Fan, YR ;

Liu, WH ;

Xu, GX .

CANCER GENE THERAPY, 2005, 12 (02) :133-140

[10] The structure and function of the replication initiator protein (Rep) of pSC101: An analysis based on a novel positive-selection system for the replication-deficient mutants [J].

Fueki, T ;

Yamaguchi, K .

JOURNAL OF BIOCHEMISTRY, 2001, 130 (03) :399-405

← 1 2 3 4 5 6 7 →