Expanding the antigenic repertoire of BCG improves protective efficacy against aerosol Mycobacterium tuberculosis infection

被引：33

作者：

Palendira, U

Spratt, JM

Britton, WJ

Triccas, JA

机构：

[1] Centenary Inst Canc Med & Cell Biol, Mycobacteriol Res Grp, Newton, NSW 2042, Australia

[2] PO Royal Brisbane Hosp, Queensland Inst Med Res, Cooperat Res Ctr Vaccine Technol, Brisbane, Qld 4029, Australia

[3] Univ Sydney, Dept Med, Sydney, NSW 2006, Australia

来源：

VACCINE | 2005年 / 23卷 / 14期

基金：

英国医学研究理事会;

关键词：

tuberculosis; vaccine; recombinant BCG; secreted antigens;

D O I：

10.1016/j.vaccine.2004.10.007

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We have developed a strain of the tuberculosis vaccine Mycobacterium bovis bacille Calmette Guerin (BCG) that secretes high levels of a fusion protein comprising the immunodominant Mycobacterium tuberculosis Ag85B and ESAT-6 (BCG(85B-ES)). Mice vaccinated with BCG85B-ES were significantly better protected in the lung against aerosol infection with virulent M. tuberculosis than animals immunized with control BCG. The growth characteristic of BCG85B-ES in host tissue was identical to control BCG, suggesting the improved protective efficacy was directly related to the expression of the Ag85B-ESAT-6 fusion protein. These results suggest that BCG(85B-ES) warrants further investigation to determine its suitability to control tuberculosis in humans. (C) 2004 Elsevier Ltd. All rights reserved.

引用

收藏

页码：1680 / 1685

页数：6

相关论文

共 24 条

[1]

ANDERSEN P, 1995, J IMMUNOL, V154, P3359

[2] Disruption of the genes encoding antigen 85A and antigen 85B of Mycobacterium tuberculosis H37Rv:: Effect on growth in culture and in macrophages [J].

Armitige, LY ;

Jagannath, C ;

Wanger, AR ;

Norris, SJ .

INFECTION AND IMMUNITY, 2000, 68 (02) :767-778

[3] Virulence, immunogenicity, and protective efficacy of two recombinant Mycobacterium bovis bacillus calmette-gue'rin strains expressing the antigen ESAT-6 from Mycobacterium tuberculosis [J].

Bao, L ;

Chen, W ;

Zhang, HD ;

Wang, XY .

INFECTION AND IMMUNITY, 2003, 71 (04) :1656-1661

[4] Comparative genomics of BCG vaccines by whole-genome DNA microarray [J].

Behr, MA ;

Wilson, MA ;

Gill, WP ;

Salamon, H ;

Schoolnik, GK ;

Rane, S ;

Small, PM .

SCIENCE, 1999, 284 (5419) :1520-1523

[5] ESAT-6 subunit vaccination against Mycobacterium tuberculosis [J].

Brandt, L ;

Elhay, M ;

Rosenkrands, I ;

Lindblad, EB ;

Andersen, P .

INFECTION AND IMMUNITY, 2000, 68 (02) :791-795

[6] The growing burden of tuberculosis - Global trends and interactions with the HIV epidemic [J].

Corbett, EL ;

Watt, CJ ;

Walker, N ;

Maher, D ;

Williams, BG ;

Raviglione, MC ;

Dye, C .

ARCHIVES OF INTERNAL MEDICINE, 2003, 163 (09) :1009-1021

[7] VARIATION IN PROTECTION BY BCG - IMPLICATIONS OF AND FOR HETEROLOGOUS IMMUNITY [J].

FINE, PEM .

LANCET, 1995, 346 (8986) :1339-1345

[8] Recombinant bacillus Calmette-Guerin (BCG) vaccines expressing the Mycobacterium tuberculosis 30-kDa major secretory protein induce greater protective immunity against tuberculosis than conventional BCG vaccines in a highly susceptible animal model [J].

Horwitz, MA ;

Harth, G ;

Dillon, BJ ;

Maslesa-Galic, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (25) :13853-13858

[9] A new vaccine against tuberculosis affords greater survival after challenge than the current vaccine in the guinea pig model of pulmonary tuberculosis [J].

Horwitz, MA ;

Harth, G .

INFECTION AND IMMUNITY, 2003, 71 (04) :1672-1679

[10] The primary mechanism of attenuation of bacillus Calmette-Guerin is a loss of secreted lytic function required for invasion of lung interstitial tissue [J].

Hsu, T ;

Hingley-Wilson, SM ;

Chen, B ;

Chen, M ;

Dai, AZ ;

Morin, PM ;

Marks, CB ;

Padiyar, J ;

Goulding, C ;

Gingery, M ;

Eisenberg, D ;

Russell, RG ;

Derrick, SC ;

Collins, FM ;

Morris, SL ;

King, CH ;

Jacobs, WR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (21) :12420-12425