The expression of estrogen receptor and estrogen effect in MBA-15 marrow stromal osteoblasts

被引：16

作者：

Shamay, A ^{[1
]}

Knopov, V ^{[1
]}

Benayahu, D ^{[1
]}

机构：

[1] TEL AVIV UNIV, SACKLER SCH MED, DEPT CELL BIOL & HISTOL, IL-69978 TEL AVIV, ISRAEL

来源：

CELL BIOLOGY INTERNATIONAL | 1996年 / 20卷 / 06期

关键词：

bone marrow; osteoblasts; estrogen receptor;

D O I：

10.1006/cbir.1996.0050

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

MBA-15, a marrow stromal-derived cell line, was shown to express an estrogen receptor. This finding was confirmed by in situ hybridization and receptor binding assay. An exposure to estrogen (10(-12)-10(-6) M) in a dose response manner resulted in a decrease of cell proliferation as measured by MTT assay. Cell function was measured by enzymatic activities of two osteoblastic markers, CD10/NEP and alkaline phosphatase. These enzymatic activities were elevated following the estrogen treatment. This model enabled direct evaluation of the estrogen effect on stromal osteoblast cells. (C) 1996 Academic Press Limited

引用

页码：401 / 405

页数：5

共 25 条

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