Effects of agitation and aeration on the production of extracellular glucose oxidase from a recombinant Saccharomyces cerevisiae

被引：18

作者：

Kapat, A ^{[1
]}

Jung, JK ^{[1
]}

Park, YH ^{[1
]}

Hong, SY ^{[1
]}

Choi, HK ^{[1
]}

机构：

[1] Doosan Training & Technol Ctr, Biotechnol Grp, Yong In 449840, Kyonggi, South Korea

来源：

BIOPROCESS ENGINEERING | 1998年 / 18卷 / 05期

关键词：

D O I：

10.1007/PL00008994

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A recombinant strain of Saccharomyces cerevisiae, GAL-GO2, was developed to facilitate the production of extracellular glucose oxidase (GOD). The recombinant strain secreted 85% (8.7 U/ml) of the total GOD (10.3 U/ml) produced in shake flask culture. For further enhancement of GOD production, optimization of the speed of agitation and the rate of aeration in a stirred tank fermentor was carried out. Response surface methodology with appropriate statistical experimental design was employed for this purpose. The maximal level of extracellular GOD was achieved when the speed of agitation and the rate of aeration were 420 rpm and 0.25 vvm, respectively. The enzyme production was increased by 74% compared to the level obtained under unoptimized conditions.

引用

页码：347 / 351

页数：5

共 18 条

[1] BANKS J G, 1986, Biotechnology and Applied Biochemistry, V8, P103
[2] ON THE EXPERIMENTAL ATTAINMENT OF OPTIMUM CONDITIONS
BOX, GEP
WILSON, KB
[J]. JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES B-STATISTICAL METHODOLOGY, 1951, 13 (01) : 1 - 45
[3] MULTI-FACTOR EXPERIMENTAL-DESIGNS FOR EXPLORING RESPONSE SURFACES
BOX, GEP
HUNTER, JS
[J]. ANNALS OF MATHEMATICAL STATISTICS, 1957, 28 (01): : 195 - 241
[4] GLUCOAMYLASE AND GLUCOSE OXIDASE PREPARATIONS AND THEIR COMBINED APPLICATION FOR CONVERSION OF MALTOSE TO GLUCONIC ACID
CHO, YK
BAILEY, JE
[J]. BIOTECHNOLOGY AND BIOENGINEERING, 1977, 19 (02) : 185 - 198
[5] FERMENTATION OF A YEAST PRODUCING ASPERGILLUS-NIGER GLUCOSE-OXIDASE - SCALE-UP, PURIFICATION AND CHARACTERIZATION OF THE RECOMBINANT ENZYME
DEBAETSELIER, A
VASAVADA, A
DOHET, P
HATHI, V
DEBEUKELAER, M
ERPICUM, T
DECLERCK, L
HANOTIER, J
ROSENBERG, S
[J]. BIO-TECHNOLOGY, 1991, 9 (06): : 559 - 561
[6] DIRECT ELECTRICAL COMMUNICATION BETWEEN CHEMICALLY MODIFIED ENZYMES AND METAL-ELECTRODES .2. METHODS FOR BONDING ELECTRON-TRANSFER RELAYS TO GLUCOSE-OXIDASE AND D-AMINO-ACID OXIDASE
DEGANI, Y
HELLER, A
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1988, 110 (08) : 2615 - 2620
[7] DIRECT ELECTRICAL COMMUNICATION BETWEEN CHEMICALLY MODIFIED ENZYMES AND METAL-ELECTRODES .1. ELECTRON-TRANSFER FROM GLUCOSE-OXIDASE TO METAL-ELECTRODES VIA ELECTRON RELAYS, BOUND COVALENTLY TO THE ENZYME
DEGANI, Y
HELLER, A
[J]. JOURNAL OF PHYSICAL CHEMISTRY, 1987, 91 (06) : 1285 - 1289
[8] OPTIMIZATION OF GLUCOSE-OXIDASE PRODUCTION BY ASPERGILLUS-NIGER USING GENETIC AND PROCESS-ENGINEERING TECHNIQUES
HELLMUTH, K
PLUSCHKELL, S
JUNG, JK
RUTTKOWSKI, E
RINAS, U
[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1995, 43 (06) : 978 - 984
[9] PRODUCTION OF XYLITOL FROM D-XYLOSE BY CANDIDA-TROPICALIS - OPTIMIZATION OF PRODUCTION-RATE
HORITSU, H
YAHASHI, Y
TAKAMIZAWA, K
KAWAI, K
SUZUKI, T
WATANABE, N
[J]. BIOTECHNOLOGY AND BIOENGINEERING, 1992, 40 (09) : 1085 - 1091
[10] KAUTOLA H, 1990, APPL MICROBIOL BIOT, V33, P7

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