Coupling of proton source and sink via H+-migration along the membrane surface as revealed by double patch-clamp experiments

被引:26
作者
Antonenko, YN [1 ]
Pohl, P
机构
[1] Moscow MV Lomonosov State Univ, AN Belozersky Inst Physicochem Biol, Moscow 119899, Russia
[2] Martin Luther Univ, Fak Med, Inst Med Phys & Biophys, D-06097 Halle, Germany
关键词
gramicidin; double patch-clamp; proton lateral conductance; planar bilayer lipid membrane;
D O I
10.1016/S0014-5793(98)00590-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Long-range proton transfer along the surface of black lipid bilayers was observed between two integral membrane channels (gramicidins), one operating as a proton source, the other as a sink, by patch-clamp technique. In contrast, potassium ions were shown to equilibrate with the aqueous bulk phase before being consumed. Both channels opened and closed simultaneously only if the charge between them was carried by protons. In this case an anomalous high conductance between two patched membrane fragments was measured, each of them containing one single gramicidin channel. The coupled state disappeared when the distance between these two channels was increased above the critical value, The latter was shown to increase with the channel lifetime. Our results support the idea of the 'localized' proton coupling, in which protons that have been pumped across membranes migrate along the membrane surface to reach another membrane protein that utilizes the established pH gradient. (C) 1998 Federation of European Biochemical Societies.
引用
收藏
页码:197 / 200
页数:4
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