Large-scale production and purification of recombinant Galanthus nivalis agglutinin (GNA) expressed in the methylotrophic yeast Pichia pastoris

被引：14

作者：

Baumgartner, P

Harper, K

Raemaekers, RJM

Durieux, A

Gatehouse, AMR

Davies, HV

Taylor, MA ^{[1
]}

机构：

[1] Scottish Crop Res Inst, Qual Hlth & Nutr Grp, Dundee DD2 5DA, Scotland

[2] Univ Newcastle Upon Tyne, Dept Agr & Environm Sci, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England

[3] Inst Meurice, Unite Biotechnol, B-1070 Brussels, Belgium

来源：

BIOTECHNOLOGY LETTERS | 2003年 / 25卷 / 15期

关键词：

fed-batch culture; Galanthus nivalis agglutinin; large-scale purification; lectin; Pichia pastoris;

D O I：

10.1023/A:1025007901322

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The gene coding for agglutinin from Galanthus nivalis (GNA) was expressed in, and secreted by, the methylotrophic yeast, Pichia pastoris. Transformants of P. pastoris were selected and a process to produce and purify gram quantities of recombinant GNA was developed. GNA was secreted at approximately 80 mg l(-1) at the 200 l scale and was purified to 95% homogeneity using hydrophobic interaction chromatography. The recombinant protein was similar to the protein synthesised in plant with respect to structure and biological activity.

引用

页码：1281 / 1285

页数：5

共 7 条

[1] Large-scale production, purification, and characterisation of recombinant Phaseolus vulgaris phytohemagglutinin E-form expressed in the methylotrophic yeast Pichia Pastoris [J].