A microarray-based high throughput molecular marker genotyping method: the tagged microarray marker (TAM) approach

被引：41

作者：

Flavell, AJ ^{[1
]}

Bolshakov, VN

Booth, A

Jing, R

Russell, J

Ellis, THN

Isaac, P

机构：

[1] Univ Dundee, Scottish Crop Res Inst, Plant Res Unit, Dundee DD2 5DA, Scotland

[2] Agrogene Sa, F-77550 Moissy Cramayel, France

[3] John Innes Ctr Plant Sci Res, Dept Crop Genet, Norwich NR4 7UH, Norfolk, England

[4] Scottish Crop Res Inst, Genome Dynam Programme, Dundee DD2 5DA, Scotland

[5] Univ Dundee, Sch Life Sci, Postgenom & Mol Interact Ctr, Dundee DD1 5EH, Scotland

来源：

NUCLEIC ACIDS RESEARCH | 2003年 / 31卷 / 19期

关键词：

D O I：

10.1093/nar/gng113

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A microarray-based method has been developed for scoring thousands of DNAs for a co-dominant molecular marker on a glass slide. The approach was developed to detect insertional polymorphism of transposons and works well with single nucleotide polymorphism (SNP) markers. Biotin- terminated allele-specific PCR products are spotted unpurified onto streptavidin-coated glass slides and visualised by hybridisation of fluorescent detector oligonucleotides to tags attached to the allele- specific PCR primers. Two tagged primer oligonucleotides are used per locus and each tag is detected by hybridisation to a concatameric DNA probe labelled with multiple fluorochromes.

引用

页数：8

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