Induction of Broadly Neutralizing H1N1 Influenza Antibodies by Vaccination

被引：299

作者：

Wei, Chih-Jen ^{[1
]}

Boyington, Jeffrey C. ^{[1
]}

McTamney, Patrick M. ^{[1
]}

Kong, Wing-Pui ^{[1
]}

Pearce, Melissa B. ^{[2
]}

Xu, Ling ^{[1
]}

Andersen, Hanne ^{[3
]}

Rao, Srinivas ^{[1
]}

Tumpey, Terrence M. ^{[2
]}

Yang, Zhi-Yong ^{[1
]}

Nabel, Gary J. ^{[1
]}

机构：

[1] NIAID, Vaccine Res Ctr, NIH, Bethesda, MD 20892 USA

[2] Ctr Dis Control & Prevent, Natl Ctr Immunizat & Resp Dis, Influenza Div, Atlanta, GA 30333 USA

[3] BIOQUAL, Rockville, MD 20850 USA

来源：

SCIENCE | 2010年 / 329卷 / 5995期

关键词：

RECOMBINANT ADENOVIRAL VECTORS; VIRUS; IMMUNIZATION; IMMUNOGENICITY; IMMUNITY; HIV-1;

D O I：

10.1126/science.1192517

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The rapid dissemination of the 2009 pandemic influenza virus underscores the need for universal influenza vaccines that elicit protective immunity to diverse viral strains. Here, we show that vaccination with plasmid DNA encoding H1N1 influenza hemagglutinin (HA) and boosting with seasonal vaccine or replication-defective adenovirus 5 vector encoding HA stimulated the production of broadly neutralizing influenza antibodies. This prime/boost combination increased the neutralization of diverse H1N1 strains dating from 1934 to 2007 as compared to either component alone and conferred protection against divergent H1N1 viruses in mice and ferrets. These antibodies were directed to the conserved stem region of HA and were also elicited in nonhuman primates. Cross-neutralization of H1N1 subtypes elicited by this approach provides a basis for the development of a universal influenza vaccine for humans.

引用

页码：1060 / 1064

页数：5

共 18 条

[1] Heterosubtypic neutralizing antibodies are produced by individuals immunized with a seasonal influenza vaccine [J].

Corti, Davide ;

Suguitan, Amorsolo L., Jr. ;

Pinna, Debora ;

Silacci, Chiara ;

Fernandez-Rodriguez, Blanca M. ;

Vanzetta, Fabrizia ;

Santos, Celia ;

Luke, Catherine J. ;

Torres-Velez, Fernando J. ;

Temperton, Nigel J. ;

Weiss, Robin A. ;

Sallusto, Federica ;

Subbarao, Kanta ;

Lanzavecchia, Antonio .

JOURNAL OF CLINICAL INVESTIGATION, 2010, 120 (05) :1663-1673

[2] Seasonal Influenza Vaccine Provides Priming For A/H1N1 Immunization [J].

Del Giudice, Giuseppe ;

Stittelaar, Koert J. ;

van Amerongen, Geert ;

Simon, James ;

Osterhaus, Albert D. M. E. ;

Stohr, Klaus ;

Rappuoli, Rino .

SCIENCE TRANSLATIONAL MEDICINE, 2009, 1 (12)

[3] The rational design of an AIDS vaccine [J].

Douek, DC ;

Kwong, PD ;

Nabell-, GJ .

CELL, 2006, 124 (04) :677-681

[4] Antibody Recognition of a Highly Conserved Influenza Virus Epitope [J].

Ekiert, Damian C. ;

Bhabha, Gira ;

Elsliger, Marc-Andre ;

Friesen, Robert H. E. ;

Jongeneelen, Mandy ;

Throsby, Mark ;

Goudsmit, Jaap ;

Wilson, Ian A. .

SCIENCE, 2009, 324 (5924) :246-251

[5] Combinatorial antibody libraries from survivors of the Turkish H5N1 avian influenza outbreak reveal virus neutralization strategies [J].

Kashyap, Arun K. ;

Steel, John ;

Oner, Ahmet F. ;

Dillon, Michael A. ;

Swale, Ryann E. ;

Wall, Katherine M. ;

Perry, Kimberly J. ;

Faynboym, Aleksandr ;

Illhan, Mahmut ;

Horowitz, Michael ;

Horowitz, Lawrence ;

Palese, Peter ;

Bhatt, Ramesh R. ;

Lerner, Richard A. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (16) :5986-5991

[6] Vaccines with MF59 Adjuvant Expand the Antibody Repertoire to Target Protective Sites of Pandemic Avian H5N1 Influenza Virus [J].

Khurana, Surender ;

Chearwae, Wanida ;

Castellino, Flora ;

Manischewitz, Jody ;

King, Lisa R. ;

Honorkiewicz, Agnieszka ;

Rock, Michael T. ;

Edwards, Kathryn M. ;

Del Giudice, Giuseppe ;

Rappuoli, Rino ;

Golding, Hana .

SCIENCE TRANSLATIONAL MEDICINE, 2010, 2 (15) :15ra5

[7] Protective immunity to lethal challenge of the 1918 pandemic influenza virus by vaccination [J].

Kong, Wing-pui ;

Hood, Chantelle ;

Yang, Zhi-yong ;

Wei, Chih-Jen ;

Xu, Ling ;

Garcia-Sastre, Adolfo ;

Tumpey, Terrence M. ;

Nabel, Gary J. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (43) :15987-15991

[8] Priming Immunization with DNA Augments Immunogenicity of Recombinant Adenoviral Vectors for Both HIV-1 Specific Antibody and T-Cell Responses [J].

Koup, Richard A. ;

Roederer, Mario ;

Lamoreaux, Laurie ;

Fischer, Jennifer ;

Novik, Laura ;

Nason, Martha C. ;

Larkin, Brenda D. ;

Enama, Mary E. ;

Ledgerwood, Julie E. ;

Bailer, Robert T. ;

Mascola, John R. ;

Nabel, Gary J. ;

Graham, Barney S. .

PLOS ONE, 2010, 5 (02)

[9] HIV-1 and influenza antibodies: seeing antigens in new ways [J].

Kwong, Peter D. ;

Wilson, Ian A. .

NATURE IMMUNOLOGY, 2009, 10 (06) :573-578

[10] Neutralizing antibodies elicited by immunization of monkeys with DNA plasmids and recombinant adenoviral vectors expressing human immunodeficiency virus type 1 proteins [J].

Mascola, JR ;

Sambor, A ;

Beaudry, K ;

Santra, S ;

Welcher, B ;

Louder, MK ;

VanCott, TC ;

Huang, Y ;

Chakrabarti, BK ;

Kong, WP ;

Yang, ZY ;

Xu, L ;

Montefiori, DC ;

Nabel, GJ ;

Letvin, NL .

JOURNAL OF VIROLOGY, 2005, 79 (02) :771-779

← 1 2 →