Lsm proteins bind and stabilize RNAs containing 5′ poly(A) tracts

被引:43
作者
Bergman, Naomi
Moraes, Karen C. M.
Anderson, John R.
Zaric, Bozidarka
Kambach, Christian
Schneider, Robert J.
Wilusz, Carol J.
Wilusz, Jeffrey [1 ]
机构
[1] Colorado State Univ, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80525 USA
[2] Vinca Inst Nucl Sci, Lab Mol Biol & Endocrinol, Belgrade, Serbia
[3] Paul Scherrer Inst, CH-5232 Villigen, Switzerland
[4] NYU, Sch Med, Dept Microbiol, New York, NY 10016 USA
[5] Univ Vale Paraiba, IP&D, BR-12244000 Sao Jose Dos Campos, SP, Brazil
关键词
D O I
10.1038/nsmb1287
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Many orthopoxvirus messenger RNAs have an unusual nontemplated poly( A) tract of 5 to 40 residues at the 5 ' end. The precise function of this feature is unknown. Here we show that 5 ' poly( A) tracts are able to repress RNA decay by inhibiting 3 '- to- 5 ' exonucleases as well as decapping of RNA substrates. UV cross- linking analysis demonstrated that the Lsm complex associates with the 5' poly( A) tract. Furthermore, recombinant Lsm1 - 7 complex specifically binds 5' poly( A) tracts 10 to 21 nucleotides in length, consistent with the length of 5' poly( A) required for stabilization. Knockdown of Lsm1 abrogates RNA stabilization by the 5' poly( A) tract. We propose that the Lsm complex simultaneously binds the 3' and 5' ends of these unusual messenger RNAs and thereby prevents 3' - to-5' decay. The implications of this phenomenon for cellular mRNA decay are discussed.
引用
收藏
页码:824 / 831
页数:8
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