Guiding a confocal microscope by single fluorescent nanoparticles

被引：73

作者：

Cang, Hu ^{[1
]}

Xu, C. Shan ^{[1
]}

Montiel, Daniel ^{[1
]}

Yang, Haw ^{[1
]}

机构：

[1] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Dept Chem, Div Phys Biosci, Berkeley, CA 94720 USA

来源：

OPTICS LETTERS | 2007年 / 32卷 / 18期

关键词：

D O I：

10.1364/OL.32.002729

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Confocal optical microscopes offer unparalleled high sensitivity and three-dimensional (3D) imaging capability but require slow point-by-point scanning; they are inefficient for imaging moving objects. We propose a more efficient solution. Instead of indiscriminate scanning, we let the focus of the microscope pursue the object of interest such that no time is wasted on uninformative background, allowing us to visualize 3D trajectories of fluorescent nanoparticles in solution with millisecond temporal and similar to 200 nm spatial resolution. (C) 2007 Optical Society of America.

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页码：2729 / 2731

页数：3

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