Molecular cloning of the cDNA coding for the (R)-(+)-mandelonitrile lyase of Prunus amygdalus:: temporal and spatial expression patterns in flowers and mature seeds

被引:12
作者
Suelves, M [1 ]
Puigdomènech, P [1 ]
机构
[1] CSIC, Cid, Dept Mol Genet, E-08034 Barcelona, Spain
关键词
cyanogenesis; flower (gene expression); mandelonitrile lyase (in-situ hybridization); Prunus (cyanogenesis);
D O I
10.1007/s004250050414
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A gene highly expressed in the floral organs of almond (Prunus amygdalus Batsch), and coding for the cyanogenic enzyme (R)-(+)-mandelonitrile lyase (EC 4.1.2.10), has been identified and the full-length cDNA sequenced. The temporal expression pattern in maturing seeds and during floral development was analyzed by RNA blot, and the highest mRNA levels were detected in floral tissues. The spatial mRNA accumulation pattern in almond flower buds was also analyzed by in-situ hybridization. The mRNA levels were compared during seed maturation and floral development in fruit and floral samples from cultivars classified as homozygous or heterozygous for the sweet-almond trait or homozygous for the bitter trait. No correlation was found between these characteristics and levels of mandelonitrile lyase mRNA, suggesting that the presence of this protein is not the limiting factor in the production of hydrogen cyanide.
引用
收藏
页码:388 / 393
页数:6
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