Microarray applications in neuroscience

被引:66
作者
Luo, Z [1 ]
Geschwind, DH [1 ]
机构
[1] Univ Calif Los Angeles, Dept Neurol, Neurogenet Program, Sch Med, Los Angeles, CA 90095 USA
关键词
D O I
10.1006/nbdi.2001.0387
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Advances in all facets of technology from molecular biology to imaging and computational biology offer unprecedented opportunities for improving our understanding of the brain in health and disease. Oligonucleotide and cDNA microarray analysis, using a variety of "DNA chips," is a recently developed high-throughput technique that allows for tour-de-force analysis of gene expression. We review this powerful technique, developed in genetics laboratories, with reference to applications in neurologic diseases in humans and the use of animal models. The typical microarray experiment is multistaged and includes preparation or purchase of arrays, preparation of target DNA and probe, target DNA hybridization, microarray scanning, and image analysis. The power and pitfalls of this technology are discussed in the context of neuroscience paradigms. Since unprecedented amounts of data are produced from microarray experiments, bioinformatics and modeling expertise are increasingly becoming critical components of this approach. (C) 2001 Academic Press.
引用
收藏
页码:183 / 193
页数:11
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