Genetic transformation of Monascus purpureus DSM1379

被引：29

作者：

Kim, JG

Do Choi, Y

Chang, YJ

Kim, SU ^{[1
]}

机构：

[1] Seoul Natl Univ, Coll Agr & Life Sci, Sch Agr Biotechnol, Suwon 441744, South Korea

[2] Rural Dev Adm, Natl Inst Agr Biotechnol, Bioinformat Div, Suwon 441707, South Korea

[3] Kyung Hee Univ, Plant Metab Res Ctr, Yongin 449701, South Korea

来源：

BIOTECHNOLOGY LETTERS | 2003年 / 25卷 / 18期

关键词：

electroporation; glyceraldehyde-3-phosphate dehydrogenase promoter; green fluorescent protein; hygromycin B resistance; Monascus purpureus;

D O I：

10.1023/A:1025438701383

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Monascus purpureus was transformed into hygromycin B resistance with hygromycin B phosphotransferase (hph) fused to Aspergillus nidulans trpC or a putative Monascus purpureus gpd1 promoter by electroporation. Among five strains, only M. purpureus DSM1397 was a competent recipient. Normal growth and sporulation on media containing up to 500 mg hygromycin B l(-1) occurred up to five generations. Upon transformation of the strain with the green fluorescent protein gene (sgfp) as a model gene and hph as a selection marker, characteristic green fluorescence was observed under fluoromicroscopy indicating successful transformation.

引用

页码：1509 / 1514

页数：6

共 15 条

[1] EXPRESSION OF HETEROLOGOUS GENES IN SACCHAROMYCES-CEREVISIAE FROM VECTORS UTILIZING THE GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE GENE PROMOTER [J].