Validation of in vitro probabilistic tractography

被引:170
作者
Dyrby, Tim B.
Sogaard, Lise V.
Parker, Geoffrey J.
Alexander, Daniel C.
Lind, Nanna M.
Baare, William F. C.
Hay-Schmidt, Anders
Eriksen, Nina
Pakkenberg, Bente
Paulson, Olaf B.
Jelsing, Jacob
机构
[1] Copenhagen Univ Hosp, Danish Res Ctr Magnet Resonance, DK-2650 Hvidovre, Denmark
[2] Tech Univ Denmark, Intelligent Signal Proc Grp, DK-2800 Lyngby, Denmark
[3] Univ Manchester, Manchester, Lancs, England
[4] UCL, Dept Comp Sci, Ctr Med Image Comp, London, England
[5] Univ Copenhagen, Dept Expt Med, Copenhagen, Denmark
[6] Univ Copenhagen, Dept Neurosci & Pharamacol, Copenhagen, Denmark
[7] Copenhagen Univ Hosp, Res Lab Stereol & Neurosci, Bispebjerg, Denmark
[8] Copenhagen Univ Hosp, Neurobiol Res Unit, Copenhagen, Denmark
[9] Copenhagen Univ Hosp, Dept Neurol, Mem Disorders Res Grp, Copenhagen, Denmark
基金
英国医学研究理事会;
关键词
brain; diffusion weighted imaging; Gottingen minipig; manganese; multi-fiber; neuron tracing; post mortem;
D O I
10.1016/j.neuroimage.2007.06.022
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Diffusion weighted imaging (DWI) and tractography allow the non-invasive study of anatomical brain connectivity. However, a gold standard for validating tractography of complex connections is lacking. Using the porcine brain as a highly gyrated brain model, we quantitatively and qualitatively assessed the anatomical validity and reproducibility of in vitro multi-fiber probabilistic tractography against two invasive tracers: the histochemically detectable biotinylated dextran amine and manganese enhanced magnetic resonance imaging. Post mortern DWI was used to ensure that most of the sources known to degrade the anatomical accuracy of in vivo DWI did not influence the tracking results. We demonstrate that probabilistic tractography reliably detected specific pathways. Moreover, the applied model allowed identification of the limitations that are likely to appear in many of the current tractography methods. Nevertheless, we conclude that DWI tractography can be a precise tool in studying anatomical brain connectivity. (C) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码:1267 / 1277
页数:11
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