Purification and characterization of an alkaline cellulase from a newly isolated alkalophilic Bacillus sp HSH-810

被引：95

作者：

Kim, JY

Hur, SH

Hong, JH ^{[1
]}

机构：

[1] Inje Univ, Sch Food & Life Sci, Gimhae 621749, South Korea

[2] Inje Univ, Genome Res Ctr, Gimhae 621749, South Korea

[3] Dong Eui Inst Technol, Div Food & Biotechnol, Pusan 614715, South Korea

来源：

BIOTECHNOLOGY LETTERS | 2005年 / 27卷 / 05期

关键词：

alkaline cellulase; alkalophilic; Bacillus sp; characterization; purification;

D O I：

10.1007/s10529-005-0685-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

An alkaline cellulase from Bacillus sp. HSH-810 was purified 8.7-fold with a 30% yield and a specific activity of 71 U mg(-1) protein. It was optimally active at pH 10 and 50 degrees C and was stable from pH 6 to 10 with more than 60% activity remaining after heating at 60 degrees C for 60 min. The molecular mass of cellulase was 80 kDa. It was inhibited by 50% by Fe3+ (1 mM) and Mn2+(0.1 mM) but was relatively insensitive to Hg2+ and Pb2+ at 1 mM.

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收藏

页码：313 / 316

页数：4

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