Progenitor cell isolation with a high-capacity quadrupole magnetic flow sorter

被引：49

作者：

Moore, LR

Rodriguez, AR

Williams, PS

McCloskey, K

Bolwell, BJ

Nakamura, M

Chalmers, JJ

Zborowski, M

机构：

[1] Cleveland Clin Fdn, Dept Biomed Engn ND20, Cleveland, OH 44195 USA

[2] Cleveland Clin Fdn, Bone Marrow Transplantat Program, Cleveland, OH 44195 USA

[3] Ohio State Univ, Dept Chem Engn, Koffolt Labs 121, Columbus, OH 43210 USA

来源：

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS | 2001年 / 225卷 / 1-2期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

magnetic cell separation; immunomagnetic separation; cell sorting quadrupole separator; magnetic particle; progenitor cell; CD34+;

D O I：

10.1016/S0304-8853(00)01251-8

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Rapid and efficient sorting is important for progenitor cell isolation. Therefore, we have built and evaluated a high-speed, continuous flow, quadrupole magnetic sorter. The cross-over behavior of non-magnetic particles, combined with separation theory is used to optimize performance. CD34 + progenitor cells are separated to purities from 64-95% and a throughput of 10(7) cells/s. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：277 / 284

页数：8

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