Human cytomegalovirus UL69 protein induces cells to accumulate in G1 phase of the cell cycle

被引:91
作者
Lu, MS [1 ]
Shenk, T [1 ]
机构
[1] Princeton Univ, Howard Hughes Med Inst, Dept Mol Biol, Princeton, NJ 08544 USA
关键词
D O I
10.1128/JVI.73.1.676-683.1999
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Earlier studies have revealed that human cytomegalovirus rapidly inhibits the growth of fibroblasts, blocking cell cycle progression at multiple points, including the G(1)-to-S-phase transition. The present study demonstrates that the UL69 protein, a virus-encoded constituent of the virion, is able to arrest cell cycle progression when introduced into uninfected cells, Expression of the UL69 protein causes U2 OS cells and primary human fibroblasts to accumulate within the G(1) compartment of the cell cycle, and serum fails to induce the progression of quiescent human fibroblasts into the S phase when the protein is present. Therefore, the UL69 protein is at least partially responsible for the cell cycle block that is instituted after infection of permissive cells with human cytomegalovirus.
引用
收藏
页码:676 / 683
页数:8
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