Effect of electric field on liquid chromatographic separation of peptide digests - Combining capillary separation techniques

被引：41

作者：

Apffel, A

Yin, HF

Hancock, WS

McManigill, D

Frenz, J

Wu, SL

机构：

[1] Hewlett Packard Labs, Palo Alto, CA 94304 USA

[2] Genentech Inc, S San Francisco, CA 94080 USA

[3] Scios Nova Inc, Mt View, CA 94043 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 1999年 / 832卷 / 1-2期

关键词：

electric field; capillary electrochromatography; peptides;

D O I：

10.1016/S0021-9673(98)00957-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A system is described which allows operation of a range of capillary based liquid phase separations including capillary electrophoresis, isocratic and gradient capillary electrochromatography, isocratic and gradient capillary liquid chromatography and electrically assisted gradient capillary liquid chromatography. The system was coupled to electrospray ionization mass spectrometry in the electrically assisted capillary liquid chromatography mode to investigate the effect of applied voltage on the selectivity in peptide mapping separations. Analyses were performed on tryptic digests of recombinant human growth hormone and tissue plasminogen activator. The results show a small but useful effect on selectivity that can be used to fine tune specific separations. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：149 / 163

页数：15

共 11 条

[1]

Aguilar MI, 1996, METHOD ENZYMOL, V270, P3

[2] ENHANCED SENSITIVITY FOR PEPTIDE-MAPPING WITH ELECTROSPRAY LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY IN THE PRESENCE OF SIGNAL SUPPRESSION DUE TO TRIFLUOROACETIC ACID-CONTAINING MOBILE PHASES [J].