Transcription of the groESL operon in Pseudomonas aeruginosa PAO1

被引：14

作者：

Fujita, M ^{[1
]}

Amemura, A

Aramaki, H

机构：

[1] Natl Inst Genet, Radioisotope Ctr, Mishima, Shizuoka 411, Japan

[2] Fukuyama Univ, Fac Engn, Dept Biotechnol, Fukuyama, Hiroshima 72902, Japan

[3] Daiichi Coll Pharmaceut Sci, Minami Ku, Fukuoka 815, Japan

来源：

FEMS MICROBIOLOGY LETTERS | 1998年 / 163卷 / 02期

关键词：

Pseudomonas aeruginosa; groESL; transcription; heat shock;

D O I：

10.1111/j.1574-6968.1998.tb13051.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Northern hybridization and S1 nuclease mapping demonstrated that the groES and groEL genes in Pseudomonas aeruginosa were transcribed as a bicistronic mRNA of 2.2 kb. Two transcription start sites and a transcription termination site were mapped. Overlapping consensus sequences for sigma(32) and sigma(70)-dependent promoters were found in the upstream region of groES. Levels of groESL-specific mRNA were increased about 2-fold upon heat shock. This response differs from the dramatic enhancement (more than 10-fold) of groESL transcription after heat shock observed in other bacterial species. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：237 / 242

页数：6

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