Potential of human dental stem cells in repairing the complete transection of rat spinal cord

被引:101
作者
Yang, Chao [1 ,2 ]
Li, Xinghan [1 ,2 ,3 ]
Sun, Liang [1 ,2 ]
Guo, Weihua [1 ,2 ,4 ]
Tian, Weidong [1 ,2 ,3 ]
机构
[1] Sichuan Univ, West China Hosp Stomatol, Natl Engn Lab Oral Regenerat Med, Chengdu, Sichuan, Peoples R China
[2] Sichuan Univ, West China Hosp Stomatol, State Key Lab Oral Dis, Chengdu, Sichuan, Peoples R China
[3] Sichuan Univ, West China Stomatol, Dept Oral & Maxillofacial Surg, Chengdu, Sichuan, Peoples R China
[4] Sichuan Univ, West China Stomatol, Dept Pediat Dent, Chengdu, Sichuan, Peoples R China
基金
中国国家自然科学基金;
关键词
dental stem cells; cell pellets; spinal cord injury; complete transection; cell therapy; PROGENITOR CELLS; IN-VITRO; AXONAL REGENERATION; HUMAN BRAIN; INJURY; SURVIVAL; GROWTH; OLIGODENDROCYTES; TRANSPLANTATION; INHIBITION;
D O I
10.1088/1741-2552/aa596b
中图分类号
R318 [生物医学工程];
学科分类号
100103 [病原生物学];
摘要
Objective. The adult spinal cord of mammals contains a certain amount of neural precursor cells, but these endogenous cells have a limited capacity for replacement of lost cells after spinal cord injury. The exogenous stem cells transplantation has become a therapeutic strategy for spinal cord repairing because of their immunomodulatory and differentiation capacity. In addition, dental stem cells originating from the cranial neural crest might be candidate cell sources for neural engineering. Approach. Human dental follicle stem cells (DFSCs), stem cells from apical papilla (SCAPs) and dental pulp stem cells (DPSCs) were isolated and identified in vitro, then green GFP-labeled stem cells with pellets were transplanted into completely transected spinal cord. The functional recovery of rats and multiple neuro-regenerative mechanisms were explored. Main results. The dental stem cells, especially DFSCs, demonstrated the potential in repairing the completely transected spinal cord and promote functional recovery after injury. The major involved mechanisms were speculated below: First, dental stem cells inhibited the expression of interleukin-1 beta to reduce the inflammatory response; second, they inhibited the expression of ras homolog gene family member A (RhoA) to promote neurite regeneration; third, they inhibited the sulfonylurea receptor1 (SUR-1) expression to reduce progressive hemorrhagic necrosis; lastly, parts of the transplanted cells survived and differentiated into mature neurons and oligodendrocytes but not astrocyte, which is beneficial for promoting axons growth. Significance. Dental stem cells presented remarkable tissue regenerative capability after spinal cord injury through immunomodulatory, differentiation and protection capacity.
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页数:14
相关论文
共 46 条
[1]
Mesenchymal-Stem-Cell-Induced Immunoregulation Involves FAS-Ligand-/FAS-Mediated T Cell Apoptosis [J].
Akiyama, Kentaro ;
Chen, Chider ;
Wang, DanDan ;
Xu, Xingtian ;
Qu, Cunye ;
Yamaza, Takayoshi ;
Cai, Tao ;
Chen, WanJun ;
Sun, Lingyun ;
Shi, Songtao .
CELL STEM CELL, 2012, 10 (05) :544-555
[2]
Arnold S A, 2011, J NEUROTRAUMA
[3]
Implanted Adult Human Dental Pulp Stem Cells Induce Endogenous Axon Guidance [J].
Arthur, Agnieszka ;
Shi, Songtao ;
Zannettino, Andrew C. W. ;
Fujii, Nobutaka ;
Gronthos, Stan ;
Koblar, Simon A. .
STEM CELLS, 2009, 27 (09) :2229-2237
[4]
Absence of IL-1β positively affects neurological outcome, lesion development and axonal plasticity after spinal cord injury [J].
Boato, Francesco ;
Rosenberger, Karen ;
Nelissen, Sofie ;
Geboes, Lies ;
Peters, Eva M. ;
Nitsch, Robert ;
Hendrix, Sven .
JOURNAL OF NEUROINFLAMMATION, 2013, 10
[5]
Surface characterization of the extracellular matrix remaining after cell detachment from a thermoresponsive polymer [J].
Canavan, HE ;
Cheng, XH ;
Graham, DJ ;
Ratner, BD ;
Castner, DG .
LANGMUIR, 2005, 21 (05) :1949-1955
[6]
Fibrinogen as a key regulator of inflammation in disease [J].
Davalos, Dimitrios ;
Akassoglou, Katerina .
SEMINARS IN IMMUNOPATHOLOGY, 2012, 34 (01) :43-62
[7]
Survival, Differentiation, and Neuroprotective Mechanisms of Human Stem Cells Complexed With Neurotrophin-3-Releasing Pharmacologically Active Microcarriers in an Ex Vivo Model of Parkinson's Disease [J].
Daviaud, Nicolas ;
Garbayo, Elisa ;
Sindji, Laurence ;
Martinez-Serrano, Alberto ;
Schiller, Paul C. ;
Montero-Menei, Claudia N. .
STEM CELLS TRANSLATIONAL MEDICINE, 2015, 4 (06) :670-684
[8]
Dergham P, 2002, J NEUROSCI, V22, P6570
[9]
Rho kinase inhibition enhances axonal regeneration in the injured CNS [J].
Fournier, AE ;
Takizawa, BT ;
Strittmatter, SM .
JOURNAL OF NEUROSCIENCE, 2003, 23 (04) :1416-1423
[10]
Reactions of oligodendrocytes to spinal cord injury: Cell survival and myelin repair [J].
Frei, E ;
Klusman, I ;
Schnell, L ;
Schwab, ME .
EXPERIMENTAL NEUROLOGY, 2000, 163 (02) :373-380