A microfabricated biosensor for detecting foodborne bioterrorism agents

被引:69
作者
Radke, SM [1 ]
Alocilja, EC [1 ]
机构
[1] Michigan State Univ, Dept Biosyst Engn, E Lansing, MI 48824 USA
关键词
biological cells; biomedical transducers; impedance; microelectrodes;
D O I
10.1109/JSEN.2005.848138
中图分类号
TM [电工技术]; TN [电子技术、通信技术];
学科分类号
0808 ; 0809 ;
摘要
A bioseiisor for the detection of pathogenic bacteria was developed for biosecurity applications. The sensor was fabricated using photolithography and incorporates heterobifunctional crosslinkers and immobilized antibodies. The sensor detected the change in impedance caused by the presence of bacteria immobilized on interifigitated gold electrodes and was fabricated from (100) silicon with a 2-mu m layer of thermal oxide as an insulating layer. The sensor has a large active area of 9.6 mm(2) and consists of two interdigital gold electrode arrays each measuring 0.8 x 6 mm. Pathogenic Escherichia coli and Salmonella infantis were tested in serially diluted pure culture. Analyte specific antibodies were immobilized to the oxide between the electrodes to create a biological sensing surface. After immersing the biosensor in solution, the impedance across the interdigital electrodes was measured. Bacteria cells present in the sample solution attached to the antibodies and became tethered to the electrode array thereby causing a change in measured impedance. The biosensor was able to discriminate between different cellular concentrations from 10(4) - 10(7) CFU/mL (colony-forming units per milliliter) in solution. The sample testing process, including data acquisition, required 5 min. The design, fabrication, and testing of the biosensor is discussed along with the implications of these findings toward further biosensor development.
引用
收藏
页码:744 / 750
页数:7
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