Modulation of immunostimulatory activity of CpG oligonucleotides by site-specific deletion of nucleobases

被引：45

作者：

Yu, D ^{[1
]}

Kandimalla, ER ^{[1
]}

Zhao, QY ^{[1
]}

Cong, YP ^{[1
]}

Agrawal, S ^{[1
]}

机构：

[1] Hybridon Inc, Cambridge, MA 02139 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2001年 / 11卷 / 17期

关键词：

D O I：

10.1016/S0960-894X(01)00418-8

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The effect of nucleobase deletion in the 3 '- or the 5 ' -flanking sequence to a CpG-motif on immunostimulatory activity of CpG-containing oligonucleotides was examined by cell proliferation, secretion of IL-12 and IL-6 in mouse spleen cell cultures, and by spleen enlargement in mice. Deletion of one or two nucleobases in the 3 ' -flanking sequence to a CpG-motif at certain positions did not affect immunostimulatory activity, while similar deletions in the 5 ' -flanking sequence increased immunostimulatory activity compared with the parent oligo. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：2263 / 2267

页数：5

共 14 条

[1] CpG DNA overcomes hyporesponsiveness to hepatitis B vaccine in orangutans [J].