Detection of a phytoplasma associated with grapevine Flavescence doree in Clematis vitalba

被引：46

作者：

Angelini, E ^{[1
]}

Squizzato, F ^{[1
]}

Lucchetta, G ^{[1
]}

Borgo, M ^{[1
]}

机构：

[1] Ist Sperimentale Viticoltura, I-31015 Conegliano, TV, Italy

来源：

EUROPEAN JOURNAL OF PLANT PATHOLOGY | 2004年 / 110卷 / 02期

关键词：

DNA sequence; elm yellows group; grapevine yellows; PCR; RFLP;

D O I：

10.1023/B:EJPP.0000015361.95661.37

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

About 40 different species of wild herbaceous and woody plants were collected in underbrush close to a vineyard where Flavescence doree (FD) has been reported for several years. Polymerase chain reaction assays were carried out using DNA extracted from leaves of each species for the detection of the presence of phytoplasmas. Only samples of Clematis vitalba were found to be infected with phytoplasmas. Restriction fragment length polymorphism and sequencing data of the 16S ribosomal RNA gene and of a non-ribosomal DNA fragment FD9 revealed that the phytoplasma isolate was identical to that causing FD in the nearby vineyard. The isolate identified in the clematis is the same as the FD-C phytoplasma found in grapevine in north-east Italy.

引用

页码：193 / 201

页数：9

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