White cell subsets in apheresis and filtered platelet concentrates

被引:36
作者
Sowemimo-Coker, SO
Kim, A
Tribble, E
Brandwein, HJ
Wenz, B
机构
[1] Pall Corp, Sci Serv, Port Washington, NY 11050 USA
[2] Pall Corp, Lab Serv, Port Washington, NY 11050 USA
关键词
D O I
10.1046/j.1537-2995.1998.38798346633.x
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
BACKGROUND: White cell (WBC)-reduced platelet concentrates (PCs) are defined by their absolute WBC count, a criterion which provides no information regarding the various WBC subsets contained in the PC. These heterogeneous cells are known to mediate different physiologic and pathophysiologic functions and account for distinct adverse transfusion responses.This study describes a method which allows the detection and quantification of these subsets and characterizes their presence in a variety of platelet components. STUDY DESIGN AND METHODS: Random-donor pooled PCs (RD PCs) and single-donor apheresis PCs (SD PCs) were studied. RD PCs consisting of 6 units of 2- to 3-day old PCs were randomly assigned to be filtered with one of four WBC-reduction filters from three different manufacturers (n = 34). The residual WBCs were pelleted by centrifugation and isolated on a density gradient. The various WBC subsets were quantified by flow cytometry in unfiltered and filtered PCs using fluorescence and two-angle light scatter. SD PCs obtained with two manufacturer's systems and three processing protocols (n = 30) were studied in like manner. RESULTS: WBC counts for non-WBC-reduced PCs averaged 3 x 10(8) in RD PCs and ranged from 8.6 to 9.6 x 10(6) per SD PC. Residual WBC counts in filtered PCs ranged from 2.3 x 10(4) to 2.2 x 10(5) and those in WBC-reduced SD PCs averaged 2.2 x 105 per unit. The data demonstrate significant phenotypic differences among PCs produced with various procedures. All SD PCs and two of four filtered RD PCs contained five WBC populations including granulocytes and monocytes, while RD PCs filtered with the remaining manufacturer's devices contained only lymphocytes. CONCLUSION: The data confirm that distinct phenotypic differences exist among PCs prepared with differ ent devices and/or procedures. It is suggested that as for non-generic pharmaceuticals, the clinical benefits of these various PCs should be individually proved.
引用
收藏
页码:650 / 657
页数:8
相关论文
共 30 条
[1]  
[Anonymous], LANCET
[2]   Allogeneic blood transfusions, immunomodulation, and postoperative bacterial infection: Do we have the answers yet? [J].
Blajchman, MA .
TRANSFUSION, 1997, 37 (02) :121-125
[3]   A COMPARISON OF FILTERED LEUKOCYTE-REDUCED AND CYTOMEGALOVIRUS (CMV) SERONEGATIVE BLOOD PRODUCTS FOR THE PREVENTION OF TRANSFUSION-ASSOCIATED CMV INFECTION AFTER MARROW TRANSPLANT [J].
BOWDEN, RA ;
SLICHTER, SJ ;
SAYERS, M ;
WEISDORF, D ;
CAYS, M ;
SCHOCH, G ;
BANAJI, M ;
HAAKE, R ;
WELK, K ;
FISHER, L ;
MCCULLOUGH, J ;
MILLER, W .
BLOOD, 1995, 86 (09) :3598-3603
[4]  
BRYTTING M, 1995, TRANSPLANTATION, V60, P961
[5]   GRANULOCYTE TRANSFUSION THERAPY AND AMPHOTERICIN-B - ADVERSE REACTIONS [J].
DUTCHER, JP ;
KENDALL, J ;
NORRIS, D ;
SCHIFFER, C ;
AISNER, J ;
WIERNIK, PH .
AMERICAN JOURNAL OF HEMATOLOGY, 1989, 31 (02) :102-108
[6]  
European Committee (Partial Agreement) on Blood Transfusion (CD P TS), 1992, GUID PREP US QUAL AS
[7]  
GLASSER L, 1985, BLOOD, V66, P267
[8]   THE EFFECT OF GRANULOCYTE TRANSFUSIONS ON THE INCIDENCE OF CYTOMEGALOVIRUS-INFECTION AFTER ALLOGENEIC MARROW TRANSPLANTATION [J].
HERSMAN, J ;
MEYERS, JD ;
THOMAS, ED ;
BUCKNER, CD ;
CLIFT, R .
ANNALS OF INTERNAL MEDICINE, 1982, 96 (02) :149-152
[9]  
Jacobs LB, 1996, ARCH PATHOL LAB MED, V120, P684
[10]   Randomised comparison of leucocyte-depleted versus buffy-coat-poor blood transfusion and complications after colorectal surgery [J].
Jensen, LS ;
KissmeyerNielsen, P ;
Wolff, B ;
Qvist, N .
LANCET, 1996, 348 (9031) :841-845