Transformation of Cell-Derived Microparticles into Quantum-Dot-Labeled Nanovectors for Antitumor siRNA Delivery

被引：107

作者：

Chen, Gang ^{[1
,2
,3
,4
]}

Zhu, Jun-Yi ^{[3
,4
]}

Zhang, Zhi-Ling ^{[1
,2
]}

Zhang, Wei ^{[3
,4
]}

Ren, Jian-Gang ^{[3
,4
]}

Wu, Min ^{[1
,2
]}

Hong, Zheng-Yuan ^{[1
,2
]}

Lv, Cheng ^{[1
,2
]}

Pang, Dai-Wen ^{[1
,2
]}

Zhao, Yi-Fang ^{[3
,4
]}

机构：

[1] Wuhan Univ, Key Lab Analyt Chem Biol & Med, Minist Educ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China

[2] Wuhan Univ, Inst Adv Studies, Wuhan 430072, Peoples R China

[3] Wuhan Univ, Key Lab Oral Biomed, Minist Educ, Wuhan 430079, Peoples R China

[4] Wuhan Univ, Dept Oral & Maxillofacial Surg, Sch & Hosp Stomatol, Wuhan 430079, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2015年 / 54卷 / 03期

基金：

中国博士后科学基金; 中国国家自然科学基金;

关键词：

biogenic vesicles; cell-derived microparticles; nanoparticles; quantum dots; targeted delivery; VIRUS;

D O I：

10.1002/anie.201410223

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

Cell-derived microparticles (MPs) have been recently recognized as critical intercellular information conveyors. However, further understanding of their biological behavior and potential application has been hampered by the limitations of current labeling techniques. Herein, a universal donor-cell-assisted membrane biotinylation strategy was proposed for labeling MPs by skillfully utilizing the natural membrane phospholipid exchange of their donor cells. This innovative strategy conveniently led to specific, efficient, reproducible, and biocompatible quantum dot (QD) labeling of MPs, thereby reliably conferring valuable traceability on MPs. By further loading with small interference RNA, QD-labeled MPs that had inherent cell-targeting and biomolecule-conveying ability were successfully employed for combined bioimaging and tumor-targeted therapy. This study provides the first reliable and biofriendly strategy for transforming biogenic MPs into functionalized nanovectors.

引用

页码：1036 / 1040

页数：5

共 18 条

[1]

Ultrasmall Near-Infrared Ag2Se Quantum Dots with Tunable Fluorescence for in Vivo Imaging [J].