High-performance liquid chromatographic method for the determination of juglone in fresh walnut leaves

被引：33

作者：

Girzu, M ^{[1
]}

Fraisse, D ^{[1
]}

Carnat, AP ^{[1
]}

Carnat, A ^{[1
]}

Lamaison, JL ^{[1
]}

机构：

[1] Fac Pharm, Lab Pharmacognosie & Phytotherapie, F-63100 Clermont Ferrand, France

来源：

JOURNAL OF CHROMATOGRAPHY A | 1998年 / 805卷 / 1-2期

关键词：

plant material; juglone; naphthoquinones;

D O I：

10.1016/S0021-9673(97)01300-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An efficient high-performance liquid chromatographic gradient method for the determination of juglone in fresh walnut leaves was developed and validated. The leaves were collected and extracted by maceration in chloroform for 1 h at room temperature. The chloroform solution was evaporated under reduced pressure to give a dry residue recovered by methanol. The methanol solution was chromatographed on an LiChrospher-RP 18 end-capped 125x4 mm column, with a gradient of mobile phase: water-phosphoric acid-acetonitrile; juglone was detected by visible absorbance at 420 nm, (C) 1998 Elsevier Science B.V.

引用

页码：315 / 318

页数：4

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