Beneficial effects of DL-α-lipoic acid on cyclophosphamide-induced oxidative stress in mitochondrial fractions of rat testis

被引:54
作者
Selvakumar, E [1 ]
Prahalathan, C [1 ]
Mythili, Y [1 ]
Varalakshmi, P [1 ]
机构
[1] Univ Madras, Dr ALM Post Grad Inst Basic Med Sci, Dept Biochem Med, Madras 600113, Tamil Nadu, India
关键词
cyclophosphamide; lipoic acid; testis; mitochondria; antioxidant; lipid peroxidation;
D O I
10.1016/j.cbi.2005.01.009
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The present study investigated the protective efficacy Of (DL)-alpha-lipoic acid on the peroxidative damage and abnormal antioxidant levels in the mitochondrial fraction of testis in cyclophosphamide (CP) administered rats. Male Wistar rats of 140 20 g were categorized into four groups. Two groups were administered CP (15 mg/kg body weight once a week for 10 weeks by oral gavage) to induce testicular toxicity; one of these groups received lipoic acid treatment (35 mg/kg body weight intraperitoneally once a week for 10 weeks, 24 h prior to CP administration). A vehicle-treated control group and a lipoic acid drug control group were also included. The mitochondrial fraction of untreated CP-exposed testis showed 1.84-fold increase in lipid peroxidation, along with a significant (P < 0.001) increase in hydrogen peroxide levels. In CP-exposed rats, we observed abnormal changes in the activities/levels of mitochondrial enzymic (superoxide dismutase, glutathione peroxidase and glutathione reductase) and nonenzymic (reduced glutathione, ascorbate and alpha-tocopherol) antioxidants. CP-treated rats also showed decline in the activities of mitochondrial enzymes such as succinate dehydrogenase, malate dehydrogenase and isocitrate dehydrogenase. In contrast, rats pretreated with lipoic acid showed normal lipid peroxidation and antioxidant defenses, thereby showing the protection rendered by lipoic acid. (c) 2005 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:59 / 66
页数:8
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