Molecular markers associated with T and Z, two genes controlling partly colored seed coat patterns in common bean

Five genes control the expression of partly colored seed coat patterns in common bean (Phaseolus vulgaris L.), viz., T,Z, L, Bip, and J.Because these genes have complex interactions, giving various seed coat patterns, it is often difficult to assign specific effects to a particular gene. The problem is compounded by multiple alleles at T,:Z, and L. Molecular markers linked to those pattern genes have the potential to assist in identifying the genes involved in specific interactions. We have begun a series of experiments to identify useful molecular markers linked to these genes. A group of three parental genotypes and five backcross lines were screened by random amplified polymorphic DNA (RAPD) techniques to identify putative markers. Potential markers were then mapped in appropriate BC3-F-2 populations. To date, recombination has not been detected between RAPD markers OAM13(1350), and OM19(400) and the T gene. A single recombinant was detected between RAPD marker OAM10(560) and the Z gene, and the marker mapped at a distance of 1.4 centimorgans (cM).