Two additional components of the accessory sec system mediating export of the Streptococcus gordonii platelet-binding protein GspB

被引:51
作者
Takamatsu, D
Bensing, BA
Sullam, PM
机构
[1] Vet Affairs Med Ctr, Dept Med, San Francisco, CA 94121 USA
[2] Univ Calif San Francisco, San Francisco, CA 94143 USA
关键词
D O I
10.1128/JB.187.11.3878-3883.2005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The gspB-secY2A2 locus of Streptococcus gordonii strain M99 encodes the platelet-binding glycoprotein GspB, along with proteins that mediate its glycosylation and export. We have identified two additional components of the accessory Sec system (Asp4 and Asp5) encoded just downstream of gtfB in the gspB-secY2A2 locus. These proteins are required for GspB export and for normal levels of platelet binding by M99. Asp4 and Asp5 may be functional homologues of SecE and SecG, respectively.
引用
收藏
页码:3878 / 3883
页数:6
相关论文
共 15 条
[1]   The Streptococcus gordonii surface proteins GspB and Hsa mediate binding to sialylated carbohydrate epitopes on the platelet membrane glycoprotein Ibα [J].
Bensing, BA ;
López, JA ;
Sullam, PA .
INFECTION AND IMMUNITY, 2004, 72 (11) :6528-6537
[2]   The Streptococcus gordonii platelet binding protein GspB undergoes glycosylation independently of export [J].
Bensing, BA ;
Gibson, BW ;
Sullam, PM .
JOURNAL OF BACTERIOLOGY, 2004, 186 (03) :638-645
[3]   An accessory sec locus of Streptococcus gordonii is required for export of the surface protein GspB and for normal levels of binding to human platelets [J].
Bensing, BA ;
Sullam, PM .
MOLECULAR MICROBIOLOGY, 2002, 44 (04) :1081-1094
[4]   Improved vectors for nisin-controlled expression in gram-positive bacteria [J].
Bryan, EM ;
Bae, T ;
Kleerebezem, H ;
Dunny, GM .
PLASMID, 2000, 44 (02) :183-190
[5]   ISOLATION AND CHARACTERIZATION OF THE SECE HOMOLOG GENE OF BACILLUS-SUBTILIS [J].
JEONG, SM ;
YOSHIKAWA, H ;
TAKAHASHI, H .
MOLECULAR MICROBIOLOGY, 1993, 10 (01) :133-142
[6]   The Sec protein-translocation pathway [J].
Mori, H ;
Ito, K .
TRENDS IN MICROBIOLOGY, 2001, 9 (10) :494-500
[7]  
Sambrook J, 1989, MOL CLONING LAB MANU
[9]   MECHANISMS OF PLATELET-AGGREGATION BY VIRIDANS GROUP STREPTOCOCCI [J].
SULLAM, PM ;
VALONE, FH ;
MILLS, J .
INFECTION AND IMMUNITY, 1987, 55 (08) :1743-1750
[10]   Functional analysis of the Streptococcus gordonii DL1 sialic acid-binding adhesin and its essential role in bacterial binding to platelets [J].
Takahashi, Y ;
Yajima, A ;
Cisar, JO ;
Konishi, K .
INFECTION AND IMMUNITY, 2004, 72 (07) :3876-3882