Purification and characterization of a novel bacteriocin produced by Enterococcus faecalis strain RJ-11

被引：88

作者：

Yamamoto, Y ^{[1
]}

Togawa, Y ^{[1
]}

Shimosaka, M ^{[1
]}

Okazaki, M ^{[1
]}

机构：

[1] Shinshu Univ, Fac Text Sci & Technol, Dept Appl Biol, Ueda, Nagano 3868567, Japan

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2003年 / 69卷 / 10期

关键词：

NISIN-PRODUCING LACTOCOCCI; CLASS IIA BACTERIOCINS; LACTIC-ACID BACTERIA; BACILLUS-SUBTILIS; ENTEROCINS L50A; FAECIUM L50; CANCER RISK; BIOSYNTHESIS; PEDIOCIN; GROWTH;

D O I：

10.1128/AEM.69.10.5546-5553.2003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Lactic acid bacteria exhibiting activity against the gram-positive bacterium Bacillus subtilis were isolated from rice bran. One of the isolates, identified as Enterococcus faecalis RJ-11, exhibited a wide spectrum of growth inhibition with various gram-positive bacteria. A bacteriocin purified from culture fluid, designated enterocin RJ-11, was heat stable and was not sensitive to acid and alkaline conditions, but it was sensitive to several proteolytic enzymes. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis revealed that enterocin RJ-11 had a molecular weight of 5,000 in its monomeric form. The amino acid sequence determined for purified enterocin RJ-11 exhibited high levels of similarity to the sequences of enterocins produced by Enterococcus faecium.

引用

页码：5746 / 5753

页数：8

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