Determination of bioactive nitrile glycoside(s) in drumstick (Moringa oleifera) by reverse phase HPLC

被引：52

作者：

Shanker, Karuna ^{[1
]}

Gupta, Madan M. ^{[1
]}

Srivastava, Santosh K. ^{[1
]}

Bawankule, Dnyaneshwar U. ^{[1
]}

Pal, Anirban ^{[1
]}

Khanuja, Surnan P. S. ^{[1
]}

机构：

[1] PO CIMAP, Cent Inst Med & Aromat Plants, Lucknow 226015, Uttar Pradesh, India

来源：

FOOD CHEMISTRY | 2007年 / 105卷 / 01期

关键词：

Moringa oleifera; niaziridin; niazirin; rifampicin; bioenhancer; RP-HPLC;

D O I：

10.1016/j.foodchem.2006.12.034

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A high-performance liquid chromatographic method for the determination of novel bioactive nitrile glycosides niaziridin and niazirin in the leaves, pods and bark of Moringa oleifera is reported. Niaziridin is a bioenhancer for drugs and nutrients. The analytical conditions for reversed-phase HPLC with UV detection were as follows: column, Chromolith RP-18 e, 4.6 x 100 mm 0.5 mu m (Merck); column temperature, 25 degrees C mobile phase, a 20:80 (% v/v) mixture of acetonitrile: Phosphate buffer - pH 3.8; flow rate, 0.7 ml/min; detection at 220nm. Method precision (relative standard deviation) was 1.81% for niaziridin and 1.94% for niazirin. Niaziridin (0.015% and 0.039%) and niazirin (0.038% and 0.033%) are present in leaves and pods, respectively. Niaziridin and mazirin were not detected in the bark of M oleifera. Relatively higher amount of niazirin was present in leaves in comparisons to the pods while niaziridin content was about three times higher in the pods than the leaves of the M. oleifera. The method is robust to evaluate niaziridin and niazirin in samples from M. oleifera as well as for quality assurance of pharmacologically active standardized extract. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：376 / 382

页数：7

共 21 条

[1]

[Anonymous], 1996, INT C HARMONISATION

[2] Profiling glucosinolates and phenolics in vegetative and reproductive tissues of the multi-purpose trees Moringa oleifera L. (horseradish tree) and Moringa stenopetala L. [J].

Bennett, RN ;

Mellon, FA ;

Foidl, N ;

Pratt, JH ;

Dupont, MS ;

Perkins, L ;

Kroon, PA .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2003, 51 (12) :3546-3553

[3]

Chincholkar S B., 2000, Biocontrol Potential and Their Exploration in Crop Disease Management, V1, P49

[4]

FAIZI S, 1994, J CHEM SOC P T, V1, P3035

[5]

FAIZI S, 1994, J NAT PROD, V57, P61

[6] Insulin secretagogues from Moringa oleifera with cyclooxygenase enzyme and lipid peroxidation inhibitory activities [J].

Francis, JA ;

Jayaprakasam, B ;

Olson, LK ;

Nair, MG .

HELVETICA CHIMICA ACTA, 2004, 87 (02) :317-326

[7] An antitumor promoter from Moringa oleifera Lam. [J].

Guevara, AP ;

Vargas, C ;

Sakurai, H ;

Fujiwara, Y ;

Hashimoto, K ;

Maoka, T ;

Kozuka, M ;

Ito, Y ;

Tokuda, H ;

Nishino, H .

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 1999, 440 (02) :181-188

[8]

*ICH, 1994, INT C HARM IFPMA GEN

[9]

Khanuja S. P. S., 2005, US Patent, Patent No. 6858588

[10]

Kurma S. Rao, 1998, Indian Journal of Natural Products, V14, P3

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