Identification and characterization of a new staphylococcal enterotoxin-related putative toxin encoded by two kinds of plasmids

被引：158

作者：

Omoe, K

Hu, DL

Takahashi-Omoe, H

Nakane, A

Shinagawa, K

机构：

[1] Iwate Univ, Fac Agr, Dept Vet Microbiol, Morioka, Iwate 0208550, Japan

[2] Hirosaki Univ, Sch Med, Dept Bacteriol, Hirosaki, Aomori 0368562, Japan

[3] Natl Inst Radiol Sci, Dept Tech Support & Dev, Inage Ku, Chiba 2638555, Japan

来源：

INFECTION AND IMMUNITY | 2003年 / 71卷 / 10期

关键词：

D O I：

10.1128/IAI.71.10.6088-6094.2003

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We identified and characterized a novel staphylococcal enterotoxin-like putative toxin, which is named SER. Nucleotide sequencing analysis of the ser gene revealed that ser was most closely related to the seg gene. The ser gene product, SER, was successfully expressed as a recombinant protein in an Escherichia coli expression system, and recombinant SER (rSER) showed significant T-cell stimulation activity. The SER production in ser-harboring Staphylococcus aureus strains was confirmed by Western blot analysis using anti-rSER antibody. Moreover, ser was seen to be encoded by at least two types of plasmids. In particular, one kind of plasmid encoding the ser gene has been known as a sed- and sej-carrying pIB485-related plasmid.

引用

页码：6088 / 6094

页数：7

共 31 条

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