Effect of granulocyte colony-stimulating factor mobilization on phenotypical and functional properties of immune cells

Objective. Some phenotypic and functional properties of lymphocytes from bone marrow or peripheral blood stem cell donors were compared in a randomized study. Materials and Methods. Lymphocyte subsets were analyzed by immunocytometry in blood harvested from bone marrow donors (n = 27) and from peripheral blood stern cell donors before and after granulocyte colony-stimulating factor mobilization (n = 23) and in bone marrow and peripheral blood stem cell grafts. Results. Granulocyte colony-stimulating factor mobilization increased the blood T and B, but not NEI, lymphocyte counts. AU lymphocyte counts were similar to 10-fold higher in peripheral blood stem cell grafts than in bone marrow grafts. Analysis of CD25, CD95, HLA-DR, and CD45RA expression shows that T-cell activation level was lower after granulocyte colony-stimulating factor mobilization. Similarly, granulocyte colony-stimulating factor reduced by twofold to threefold the percentage of interferon-gamma, interleukin-2, and tumor necrosis factor-alpha -secreting cells within the NK, NK-T, and T-cell subsets and severely impaired the potential for interferon-gamma production at the single-cell level, mRNA levels of both type 1 (interferon-gamma, interleukin-2) and type 2 (interleukin-gamma, interleukinn-13) cytokines sere similar to 10-fold lower in peripheral blood stem cell grafts than in bone marrow grafts. This reduced potential of cytokine production was not associated with a preferential mobilization of so-called "suppressive" cells (CD3(+)CD4(-)CD8(-), CD3(+)CD8(+)CD56(+), or CD3(+)TCRVA24(+)CD161(+)), nor with a modulation of killer cell receptors CD161, NKB1, and CD94 expression by NK, NK-T, or T cells. Conclusion. Our data demonstrate in a randomized setting that quantitative os well as qualitative differences exist between a bone marrow and a peripheral blood stem cell graft, whose ability to produce type 1 and type 2 cytokines is impaired. (C) 2001 International Society for Experimental Hematology. Published by Elsevier Science Inc.