Binding of a neutralizing antibody to dengue virus alters the arrangement of surface glycoproteins

被引：284

作者：

Lok, Shee-Mei ^{[1
]}

Kostyuchenko, Victor ^{[1
]}

Nybakken, Grant E. ^{[2
]}

Holdaway, Heather A. ^{[1
]}

Battisti, Anthony J. ^{[1
]}

Sukupolvi-Petty, Soila ^{[3
]}

Sedlak, Dagmar ^{[1
]}

Fremont, Daved H. ^{[2
]}

Chipman, Paul R. ^{[1
]}

Roehrig, John T. ^{[4
]}

Diamond, Michael S. ^{[2
,3
,5
]}

Kuhn, Richard J. ^{[1
]}

Rossmann, Michael G. ^{[1
]}

机构：

[1] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA

[2] Washington Univ, Sch Med, Dept Pathol & Immunol, St Louis, MO 63110 USA

[3] Washington Univ, Sch Med, Dept Med, St Louis, MO 63110 USA

[4] Ctr Dis Control & Prevent, Arbovirus Dis Branch, Div Vector Borne Infect Dis, Publ Hlth Serv,US Dept Hlth & Human Serv, Ft Collins, CO 80522 USA

[5] Washington Univ, Sch Med, Dept Mol Microbiol, St Louis, MO 63110 USA

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2008年 / 15卷 / 03期

关键词：

D O I：

10.1038/nsmb.1382

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The monoclonal antibody 1A1D-2 has been shown to strongly neutralize dengue virus serotypes 1, 2 and 3, primarily by inhibiting attachment to host cells. A crystal structure of its antigen binding fragment (Fab) complexed with domain III of the viral envelope glycoprotein, E, showed that the epitope would be partially occluded in the known structure of the mature dengue virus. Nevertheless, antibody could bind to the virus at 37 degrees C, suggesting that the virus is in dynamic motion making hidden epitopes briefly available. A cryo-electron microscope image reconstruction of the virus: Fab complex showed large changes in the organization of the E protein that exposed the epitopes on two of the three E molecules in each of the 60 icosahedral asymmetric units of the virus. The changes in the structure of the viral surface are presumably responsible for inhibiting attachment to cells.

引用

页码：312 / 317

页数：6

共 46 条

[1] Biophysical characterization and vector-specific antagonist activity of domain III of the tick-borne flavivirus envelope protein [J].

Bhardwaj, S ;

Holbrook, M ;

Shope, RE ;

Barrett, ADT ;

Watowich, SJ .

JOURNAL OF VIROLOGY, 2001, 75 (08) :4002-4007

[2] Evidence of viral capsid dynamics using limited proteolysis and mass spectrometry [J].

Bothner, B ;

Dong, XF ;

Bibbs, L ;

Johnson, JE ;

Siuzdak, G .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (02) :673-676

[3] Structure of a flavivirus envelope glycoprotein in its low-pH-induced membrane fusion conformation [J].

Bressanelli, S ;

Stiasny, K ;

Allison, SL ;

Stura, EA ;

Duquerroy, S ;

Lescar, J ;

Heinz, FX ;

Rey, FA .

EMBO JOURNAL, 2004, 23 (04) :728-738

[4] Crystallography & NMR system:: A new software suite for macromolecular structure determination [J].

Brunger, AT ;

Adams, PD ;

Clore, GM ;

DeLano, WL ;

Gros, P ;

Grosse-Kunstleve, RW ;

Jiang, JS ;

Kuszewski, J ;

Nilges, M ;

Pannu, NS ;

Read, RJ ;

Rice, LM ;

Simonson, T ;

Warren, GL .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1998, 54 :905-921

[5] Monoclonal antibodies that bind to domain III of dengue virus E glycoprotein are the most efficient blockers of virus adsorption to Vero cells [J].

Crill, WD ;

Roehrig, JT .

JOURNAL OF VIROLOGY, 2001, 75 (16) :7769-7773

[6]

DAVIES DR, 1990, ANNU REV BIOCHEM, V59, P439, DOI 10.1146/annurev.biochem.59.1.439

[7] The location of asparagine-linked glycans on West Nile virions controls their interactions with CD209 (dendritic cell-specific ICAM-3 grabbing nonintegrin) [J].

Davis, Carl W. ;

Mattei, Lisa M. ;

Nguyen, Hai-Yen ;

Ansarah-Sobrinho, Camilo ;

Doms, Robert W. ;

Pierson, Theodore C. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (48) :37183-37194

[8] SPIDER and WEB: Processing and visualization of images in 3D electron microscopy and related fields [J].

Frank, J ;

Radermacher, M ;

Penczek, P ;

Zhu, J ;

Li, YH ;

Ladjadj, M ;

Leith, A .

JOURNAL OF STRUCTURAL BIOLOGY, 1996, 116 (01) :190-199

[9] DENGUE AND CHIKUNGUNYA VIRUS INFECTION IN MAN IN THAILAND, 1962-1964 .2. OBSERVATIONS ON DISEASE IN OUTPATIENTS [J].

HALSTEAD, SB ;

NIMMANNITYA, S ;

MARGIOTTA, MR .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1969, 18 (6P1) :972-+

[10]

HALSTEAD SB, 1988, SCIENCE, V239, P476, DOI 10.1126/science.3277268

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