Analysis of gene expression in lung and thymus of TCDD treated C57BL6 mice using differential display RT-PCR

被引：3

作者：

Donat, S ^{[1
]}

Abel, J ^{[1
]}

机构：

[1] Univ Dusseldorf, Dept Toxicol, Med Inst Environm Hyg, D-40225 Dusseldorf, Germany

来源：

CHEMOSPHERE | 1998年 / 37卷 / 9-12期

关键词：

D O I：

10.1016/S0045-6535(98)00252-5

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The differential display RT-PCR technique offers a means to identify genes which are regulated by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Treatment of female C57BL/6 mice with 10 mu g TCDD/kg body weight followed by DDTR-PCR analysis with a set of different primers revealed differential expression for a number of mRNAs. RNA slot blot analysis of six of these clones confirmed differential expression. Further characterization of one of these clones obtained from lung tissue demonstrated that the corresponding mRNA is about 1 kb and shows significant TCDD induced expression in Northern blot analysis. A Genbank search of this clone showed no similarity to known sequences. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：1867 / 1872

页数：6

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