Liquid chromatographic electrospray mass spectrometric analysis of Coptidis Rhizoma

被引：5

作者：

Chuang, WC ^{[1
]}

Young, DS ^{[1
]}

Liu, LK ^{[1
]}

Sheu, SJ ^{[1
]}

机构：

[1] NATL TAIWAN NORMAL UNIV,DEPT CHEM,TAIPEI 117,TAIWAN

来源：

JOURNAL OF CHROMATOGRAPHY A | 1996年 / 755卷 / 01期

关键词：

Coptids Rhizoma; protoberberine alkaloids; alkaloids;

D O I：

暂无

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Seven protoberberine alkaloids from Coptidis Rhizoma were separated on a Cosmosil 5C18-MS column (5 mu m, 250x4.6 mm I.D.) using ammonium acetate buffer (isocratic) or ammonium acetate-sodium dodecyl sulfate buffer (gradient) with UV and electrospray ionization mass spectrometric (ESI-MS) detection. Selected-ion monitoring was used to resolve the compounds in an overlapped peak and collision-induced dissociation reaction was used to obtain structural infomation of individual components. By use of this LC-UV-ESI-MS method, these protoberberine alkaloids (berberine, palmatine, coptisine, epiberberine, jatrorrhizine, columbamine and berberastine) in a crude extract of Coptidis Rhizoma can be easily separated and identified within 50 min.

引用

页码：19 / 26

页数：8

共 28 条

[1] DETERMINATION OF ISOFLAVONES USING CAPILLARY ELECTROPHORESIS IN COMBINATION WITH ELECTROSPRAY MASS-SPECTROMETRY [J].