Fatty acid ethyl ester synthesis by human liver microsomes

被引:22
作者
Treloar, T
Madden, LJ
Winter, JS
Smith, JL
deJersey, J
机构
[1] UNIV QUEENSLAND, DEPT BIOCHEM, ALCOHOL RES UNIT, ST LUCIA, QLD 4072, AUSTRALIA
[2] UNIV QUEENSLAND, ROYAL BRISBANE HOSP, DEPT SURG, LIPID METAB LAB, ST LUCIA, QLD 4072, AUSTRALIA
来源
BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM | 1996年 / 1299卷 / 02期
关键词
fatty acid ethyl ester; microsomal enzyme; ethanol metabolite; acyl-CoA hydrolase; human liver; acyl-CoA; ethanol acyltransferase; ACYL-COA HYDROLASE; RAT-LIVER; CHOLESTEROL ACYLTRANSFERASE; MYOCARDIAL METABOLITES; ETHANOL-METABOLISM; COENZYME-A; PURIFICATION; SYNTHASE; PROTEIN; CARBOXYLESTERASE;
D O I
10.1016/0005-2760(95)00199-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Fatty acid ethyl esters are a family of non-oxidative metabolites of ethanol present in many tissues after ethanol consumption. In this report we demonstrate the existence in human liver of an acyl-CoA:ethanol acyltransferase activity which may be responsible in part for the synthesis of these compounds in vivo. The effects of oleoyl-CoA and ethanol concentrations, presence or absence of bovine serum albumin and detergent, pH and enzyme concentration on this activity have been determined. Acyl-CoA:ethanol acyltransferase activity is localised in the membrane-bound fraction. Using inhibitors directed against related enzyme activities, it has been shown that the activity is not related to serine-dependent carboxylesterases or acyl-CoA:cholesterol acyltransferase, but that it may be associated with acyl-CoA hydrolase activity. We have also compared acyl-CoA:ethanol acyltransferase activity with fatty acid ethyl ester synthase activity in microsomes and cytosol from the same liver. Our data indicate that these activities an comparable in vitro (on a units/g liver basis), and suggest that both may be significant in vivo.
引用
收藏
页码:160 / 166
页数:7
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