Determination of ATP using chelation-enhanced fluorescence

被引：26

作者：

Foy, GP ^{[1
]}

Pacey, GE ^{[1
]}

机构：

[1] MIAMI UNIV,DEPT CHEM,OXFORD,OH 45056

来源：

TALANTA | 1996年 / 43卷 / 02期

关键词：

ATP; chelation-enhanced fluorescence; flow-injection analysis;

D O I：

10.1016/0039-9140(95)01729-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method for the direct determination of ATP that exhibits reasonable sensitivity, and responds to very few interferants, has been developed. The chelation-enhanced fluorescence between N-(anthracen-9'-yl methyl)tris(3-aminopropyl)amine and adenosine 5'-triphosphate is utilized in this determination. The method was tested in batch and flow-injection analysis (FIA) modes. The typical detection limit for FIA determination of ATP is 1 mu M, with a linear range of 0.5-100 ppm. A typical relative standard deviation at 20 ppm is 2.3%.

引用

页码：225 / 232

页数：8

共 13 条

[1] ULTRAVIOLET ABSORPTION SPECTRA OF ADENOSINE-5'-TRIPHOSPHATE AND RELATED 5'-RIBONUCLEOTIDES [J].