Sequence-specific DNA detection using high-affinity LNA-functionalized gold nanoparticles

被引：39

作者：

McKenzie, Fiona ^{[1
]}

Faulds, Karen ^{[1
]}

Graham, Duncan ^{[1
]}

机构：

[1] Univ Strathclyde, Dept Pure & Appl Chem, Ctr Mol Nanometrol, WestCHEM, Glasgow G1 1XL, Lanark, Scotland

来源：

SMALL | 2007年 / 3卷 / 11期

基金：

英国工程与自然科学研究理事会;

关键词：

DNA; gold; hybridization; nanoparticles; NUCLEIC-ACID;

D O I：

10.1002/smll.200700225

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The use of gold nanoparticles functionalized with alkanethiol-capped LNA/DNA chimeras in a tail-to-tail hybridization mode was investigated. The nanoparticle-functionalized systems, monitored at both 260 nm and 520 nm gave extremely sharp transitions as the duplex dissociated into single-stranded DNA. The presence of nanoparticles results in the observation of an optical signature. Gold-nanoparticle functionalization with LAN demonstrated the conjugation displaying remarkable binding affinity. The gold-nanoparticles were prepared by citrate reduction of HAuCl4. Unmodified oligonucleotides were hybridized in a 1:1 ratio. The nanoparticle-labeled duplex was prepared by mixing the target single-strand DNA with two oligonucleotide probes. It was observed that the use of LNA/DNA chimeras offers the enhanced stability of duplexes formed using gold-nanoparticle conjugates.

引用

页码：1866 / 1868

页数：3

共 18 条

[1] A fluorescence-based method for determining the surface coverage and hybridization efficiency of thiol-capped oligonucleotides bound to gold thin films and nanoparticles [J].