Transport of Ca2+ and Ca2+-Dependent Permeability Transition in the Liver and Heart Mitochondria of Rats with Different Tolerance to Acute Hypoxia

被引：24

作者：

Belosludtsev, Konstantin N. ^{[1
,2
]}

Dubinin, Mikhail V. ^{[2
]}

Talanov, Eugeny Yu. ^{[1
]}

Starinets, Vlada S. ^{[2
]}

Tenkov, Kirill S. ^{[2
]}

Zakharova, Nadezhda M. ^{[3
]}

Belosludtseva, Natalia V. ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Theoret & Expt Biophys, Inst Skaya 3, Pushchino 142290, Moscow Region, Russia

[2] Mari State Univ, Dept Biochem Cell Biol & Microbiol, Pl Lenina 1, Yoshkar Ola 424001, Mari El, Russia

[3] RAS, PSCBR, Inst Cell Biophys, Inst Skaya 3, Pushchino 142290, Moscow Region, Russia

来源：

BIOMOLECULES | 2020年 / 10卷 / 01期

基金：

俄罗斯基础研究基金会;

关键词：

hypoxia; resistance to hypoxia; mitochondria; mitochondrial calcium transport; mitochondrial calcium uniporter complex; mitochondrial Ca2+-induced permeability transition pore; cyclophilin D; ATP synthase; CALCIUM; CHANNEL; RESISTANCE; ACTIVATION; SIGNALS;

D O I：

10.3390/biom10010114

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The work examines the kinetic parameters of Ca2+ uptake via the mitochondrial calcium uniporter complex (MCUC) and the opening of the Ca2+-dependent permeability transition pore (MPT pore) in the liver and heart mitochondria of rats with high resistance (HR) and low resistance (LR) to acute hypoxia. We found that the rate of Ca2+ uptake by mitochondria of the liver and heart in HR rats is higher than that in LR rats, which is associated with a higher level of the channel-forming subunit MCU in liver mitochondria of HR rats and a lower content of the dominant-negative channel subunit MCUb in heart mitochondria of HR rats. It was shown that the liver mitochondria of HR rats are more resistant to the induction of the MPT pore than those of LR rats (the calcium retention capacity of liver mitochondria of HR rats was found to be 1.3 times greater than that of LR rats). These data correlate with the fact that the level of F0F1-ATP synthase, a possible structural element of the MPT pore, in the liver mitochondria of HR rats is lower than in LR rats. In heart mitochondria of rats of the two phenotypes, no statistically significant difference in the formation of the MPT pore was revealed. The paper discusses how changes in the expression of the MCUC subunits and the putative components of the MPT pore can affect Ca2+ homeostasis of mitochondria in animals with originally different tolerance to hypoxia and in hypoxia-induced tissue injury.

引用

页数：13

共 30 条

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