Enhancement of Taxol production and excretion in Taxus chinensis cell culture by fungal elicitation and medium renewal

被引:145
作者
Wang, CG
Wu, JY [1 ]
Mei, XG
机构
[1] Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, Kowloon, Hong Kong, Peoples R China
[2] Huazhong Univ Sci & Technol, Inst Biotechnol, Wuhan 430074, Peoples R China
关键词
D O I
10.1007/s002530000567
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
An endophytic fungus, Aspergillus niger, isolated from the inner bark of a Taxus chinensis tree, was used as an elicitor to stimulate the Taxol (paclitaxel) production in a Taxus chinensis cell suspension culture. Different elicitor doses and elicitation times were tested in a batch culture: and the highest volumetric Taxol yield was achieved when 40 mg of the fungal elicitor (carbohydrate equivalent) l(-1)' was added to the culture during the late exponential-growth phase. The elicitation resulted in a more than two-fold increase in the Taxol yield and about a six-fold increase in total secretion. The Taxol yield was further improved substantially by applying medium renewal and re-elicitation to the culture. In particular, with repeated medium renewal (in a way similar to medium perfusion) and a second elicitation of the culture, the volumetric Taxol yield was increased to 67.1 +/-7.5 mg l(-1), which was about seven times the amount obtained in the non-elicited batch culture. The Taxol productivity of the perfusion-like culture with repeated fungal elicitation was 1.5 mg l(-1) day(-1), which was about 40% higher than that of the elicitor-treated batch culture and three times the productivity of the non-elicited batch culture.
引用
收藏
页码:404 / 410
页数:7
相关论文
共 30 条
[1]   Endogenous elicitor-like effects of alginate on physiological activities of plant cells [J].
Akimoto, C ;
Aoyagi, H ;
Tanaka, H .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1999, 52 (03) :429-436
[2]  
Bringi V., 1995, U.S. Patent, Patent No. [5,407,816, 5407816]
[3]   2-PHASE AIRLIFT FERMENTER OPERATION WITH ELICITATION FOR THE ENHANCED PRODUCTION OF BENZOPHENANTHRIDINE ALKALOIDS IN CELL-SUSPENSIONS OF ESCHERICHIA-CALIFORNICA [J].
BYUN, SY ;
PEDERSEN, H .
BIOTECHNOLOGY AND BIOENGINEERING, 1994, 44 (01) :14-20
[4]  
Chang HN., 1994, BIOTECHNOLOGICAL APP, P23
[5]  
Ciddi V, 1995, BIOTECHNOL LETT, V17, P1343, DOI 10.1007/BF00189223
[6]   ELICITING SECONDARY METABOLISM IN PLANT-CELL CULTURES [J].
DICOSMO, F ;
MISAWA, M .
TRENDS IN BIOTECHNOLOGY, 1985, 3 (12) :318-322
[7]   COLORIMETRIC METHOD FOR DETERMINATION OF SUGARS AND RELATED SUBSTANCES [J].
DUBOIS, M ;
GILLES, KA ;
HAMILTON, JK ;
REBERS, PA ;
SMITH, F .
ANALYTICAL CHEMISTRY, 1956, 28 (03) :350-356
[8]   Early events in the elicitation of plant defence [J].
Ebel, J ;
Mithöfer, A .
PLANTA, 1998, 206 (03) :335-348
[9]   IMPROVED GROWTH AND TAXOL YIELD IN DEVELOPING CALLI OF TAXUS-CUSPIDATA BY MEDIUM COMPOSITION MODIFICATION [J].
FETTNETO, AG ;
MELANSON, SJ ;
SAKATA, K ;
DICOSMO, F .
BIO-TECHNOLOGY, 1993, 11 (06) :731-734
[10]   Stimulation of shikonin production by combined fungal elicitation and in situ extraction in suspension cultures of Arnebia euchroma [J].
Fu, XQ ;
Lu, DW .
ENZYME AND MICROBIAL TECHNOLOGY, 1999, 24 (5-6) :243-246