Stage-specific differences in the requirements for germline stem cell maintenance in the Drosophila ovary

被引：51

作者：

Shcherbata, Halyna R. ^{[1
]}

Ward, Ellen J. ^{[1
]}

Fischer, Karin A. ^{[1
]}

Yu, Jenn-Yah ^{[1
]}

Reynolds, Steven H. ^{[1
]}

Chen, Chun-Hong ^{[2
]}

Xu, Peizhang ^{[2
,3
,4
]}

Hay, Bruce A. ^{[2
]}

Ruohola-Baker, Hannele ^{[1
]}

机构：

[1] Univ Washington, Inst Stem Cell & Regenerat Med, Dept Biochem, Seattle, WA 98195 USA

[2] CALTECH, Div Biol, Pasadena, CA 91125 USA

[3] Univ Calif San Francisco, Howard Hughes Med Inst, San Francisco, CA 94158 USA

[4] Univ Calif San Francisco, Dept Physiol, San Francisco, CA 94158 USA

来源：

CELL STEM CELL | 2007年 / 1卷 / 06期

关键词：

D O I：

10.1016/j.stem.2007.11.007

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

In this study, we uncover a role for microRNAs (miRNAs) in Drosophila germline stem cell (GSC) maintenance. Disruption of Dicer-1 function in GSCs during adult life results in GSC loss. Surprisingly, however, loss of Dicer-1 during development does not result in a GSC maintenance defect, although a defect is seen if both Dicer-1 and Dicer-2 function are disrupted. Loss of the bantam miRNA mimics the Dicer-1 maintenance defect when induced in adult GSCs, suggesting that bantam plays a key role in GSC self-renewal. Mad, a component of the TGF-beta pathway, behaves similarly to Dicer-1: adult GSC maintenance requires Mad if it is lost during adult life, but not if it is lost during pupal development. Overall, these results show stage-specific differential sensitivity of GSC maintenance to certain perturbations and suggest that there may be a Dicer-2-dependent GSC maintenance mechanism during development that is lost in later life.

引用

页码：698 / 709

页数：12

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